Use a formic acid based decal solution and MAKE SURE the specimen does not
fix (formalin fixation) longer than 32hrs (false negatives result). Use 70%
ETOH to manage the fixation time (ie fix for 24 hours, decal for 4 hrs,
place in 70% ETOH until it gets put on the processor. Hold in 70% ETOH for
Hi Kendra-
Decal Stat from Statlab is HCl based, therefore is not appropriate for
subsequent IHC, ISH, or other molecular studies. There is lots of
peer-reviewed published data, if you need to supply rationale to your
pathologists. HCl is known to significantly reduce molecular assay
Hello all,
Recently, the Kappa/Lambda ISH has quit working on our decal specimens.
When we first validated ISH, we tested decal specimens that stained
appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial
ISH validation back in 2019, but as far as I know, we have not changed
charles.ba...@baystatehealth.org
-Original Message-
From: Titus, Jennifer
Sent: Saturday, September 19, 2020 8:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ISH policies
Would anyone be willing to share their policies and procedures for ISH,
specifically relating to kappa
Would anyone be willing to share their policies and procedures for ISH,
specifically relating to kappa and lambda and CAP checklist guidelines?
Thank you,
Jen
This message originates from the Yale New Haven Health System. The information
contained in this message may be privileged and
Good morning!
Just recently we started having an issue with a heavy blue precipitate on
our bone marrow cores. We have changed nothing in our protocol and even
did parallel testing with a reference lab. Both had the precipitate, but
to a lesser intensity.
Has anyone else been seeing this?
I am looking for any info/webinar/educational material for in situ
hybridization (ISH). We would like to bring this into our lab on the Ventana
BenchMark Ultra.
Thanks,
Heidi
Heidi Bird, HT (ASCP)
Histotechnologist
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
Phone:
That may be what you have to do, as the first day of ISH needs to be RNase
free!
When I do them, it is 10 slides at a time and we have dishes and a lab
bench set aside for RNase free procedures only. Obviously this is not
feasible in all workplaces!
I would just think about it logically. Use
Hi Folks!
I was hoping to get a little input about preparing samples for ISH.
We currently RNAse-away our tools at the time of collection, toss the tissues
into formalin, process normally and then cut them using RNAse away cleaned
microtomes/forceps on a bath of DEPC treated water (in a cleaned
We are having sporadic issues with kappa and lambda ISH on our bone marrows.
What effect does EDTA have on ISH? The same as decal?
Thanks for your help! j
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org
Has anyone experience edge affect on TMA when doing ISH?
Dana Settembre
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Would anyone care to share their ISH protocols? Especially ISH double
staining with DAB and Refine Red. I use the Leica Bond Max. I am not
having luck optimizing this protocol.
Happy Holidays!!
Thanks,
Nacaela Johnson
Histology Technician
KCCC Pathology
12000 110th St., Ste. 400
Overland
Hi Everyone,
We have some cells that we have processed and embedded in histogel (thanks to
everyone for the protocols that were sent to me a couple of weeks ago).
We are planning to do IHC and ISH on these pellets. Will the histogel
interfere with the ISH procedure at all?
Kim
Kim Merriam,
Drive, Unit E
Longmont, Colorado 80504
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Friday, June 18, 2010 7:09 AM
To: Histonet
Subject: [Histonet] ISH on histogel cell pellets
Hi
USA I= nc.
8201 East Riverside Dr. Bldg 4 Suite 100
Aust= in, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] ISH on histogel cell pellets
From: Liz Chlipala [1]...@premie= rlab.com
Date: Fri, June 18, 2010 7:46 am
To: Kim
Dear Histonetters,
I have a problem with my ISH and maybe one of you can help me.
I used probes I *know* they are working. I put the mice embryos (E 10.5)
in BM Purple for staining. Unfortunately they turned black in a short time.
They were not stained in the inside. It looks like that the
Weems,
Joyce
Gesendet: Mittwoch, 23. September 2009 19:47
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] ISH on decaled bone
We have a bone biopsy that was decaled in RDO. Of course ISH is not
working. Does anyone have advice for retrieval?
Many thanks.
Joyce
Joyce Weems
Pathology
We have a bone biopsy that was decaled in RDO. Of course ISH is not
working. Does anyone have advice for retrieval?
Many thanks.
Joyce
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
Hi Histonetters!
We have recently changed to Immunocal to decal bone marrow biopsies. Immuno
stains performed on the decaled bone marrows look good. All In-situ
hybridization negative controls, and Kappa and Lambda stains on decaled bone
marrows have a blue artifact at the edges. We use the
] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, August 11, 2009 2:36 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] ISH
Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that
Dako and Ventana have had to take their ASR Kappa.Lamda product off of
the market. I am in need
, 11. August 2009 20:36
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] ISH
Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako
and Ventana have had to take their ASR Kappa.Lamda product off of the
market. I am in need of where to go for ASR or IVD in this area
Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and
Ventana have had to take their ASR Kappa.Lamda product off of the market. I am
in need of where to go for ASR or IVD in this area!!
Thanks,
Dorothy Webb, HT
Regions Histology Technical Supervisor
651-254-2962
Of Webb,
Dorothy L
Sent: Tuesday, August 11, 2009 11:36 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] ISH
Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that
Dako and Ventana have had to take their ASR Kappa.Lamda product off of
the market. I am in need of where
We use Leica - works perfectly!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, August 11, 2009 14:36
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] ISH
Can
Hello Histonetters!
We are finding ourselves coming short on the time remaining that we can
offer ISH Probes through Ventana. We have not heard when or if ISH
(In-situ hybridization) will be offered any more through them. Does
anyone out there know of other companies that would offer ISH?
Thank
, January 26, 2009 9:20 AM
Subject: [Histonet] ISH probes
Hello Histonetters!
We are finding ourselves coming short on the time remaining that we can
offer ISH Probes through Ventana. We have not heard when or if ISH
(In-situ hybridization) will be offered any more through them. Does
anyone out
Hi listmembers,
In our lab the unstained IHC-slides are thrown away. Now one slide of each
case should be stored in an adequate manner to perform an insitu
hybridization if required in the future.
I want to store it unstained but deparaffinized and coverslipped. So that it
can be filed with the
Yes, we cannot get our Her2 ISH from Ventana.
Laurie
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Patti
Loykasek
Sent: Tuesday, September 23, 2008 1:01 PM
To: histonet
Subject: [Histonet] ISH supplies from vendors
I was wondering if anyone else has
28 matches
Mail list logo
