Re: [Histonet] Immunofluorescence staining/minimizing background staining

2012-02-01 Thread Kim Merriam
:50 PM Subject: [Histonet] Immunofluorescence staining/minimizing background staining Hi, We are performing some immunofluorescence staining on mouse lung tissue.  We are getting some nice positive staining with some of our initial antibodies (procollagen, cytokeratin). We would like to minimize

[Histonet] Immunofluorescence staining/minimizing background staining

2012-01-30 Thread Kasai, Miki (NIH/NCI) [E]
Hi, We are performing some immunofluorescence staining on mouse lung tissue. We are getting some nice positive staining with some of our initial antibodies (procollagen, cytokeratin). We would like to minimize the amount of background staining we are getting. We are titering our primary