I enjoy this forum so much, I get to use brain cells I haven't used in years!
When I saw your question about other counterstains for Iron Stain I kept
thinking quick dip in Eosin (from you H&E) because I thought I remember having
to do that in the 90's when we ran our of NFR. Histologist can be so
resourceful with the right support. If I remember correctly light Eosin can
also be used as a counterstain for GMS, some have used a very pale Hematoxylin
but I personally don't care for it because it wasn't enough contrst for me.
Tony, thanks for speaking up, it's nice to know I haven't forgot eveything with
all the automation.
Cassie Davis
Message: 6
Date: Wed, 8 Aug 2018 23:29:27 +0000
From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au>
To: "Campbell, Tasha M." <tmcam...@fmh.org>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Iron Stain
Message-ID:
<4125cb6319d24437bbd26ff12d106...@svdcmbx-mex024.nswhealth.net>
Content-Type: text/plain; charset="us-ascii"
Try your eosin stain used in your H&E or 1% Neutral red in 2% acetic acid
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
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