Melanie,
Who's your vendor for buying Novec 7000?
Maria Mejia
San Francisco, CA
Affymetrix
On Apr 17, 2013, at 10:08 AM, mwh...@mcleodhealth.org wrote:
I just wanted to clarify that we don't use Novec 7000 for freezing of
muscle tissue. We use it for other routine frozen sections on
For almost two years, we have been using- with great success- a much safer
alternative to Isopentane. It is called Novec 7000 from 3M. I am NOT a
paid endorser and these are my views only , and do not reflect in any way
on my employer. Our pathologists greatly prefer the results of a freezing
, 2013 2:17 PM
To: 'Paula Sicurello'; HistoNet
Subject: RE: [Histonet] Isopentane storage-the polling is open
We store at room temperature in a flammable cabinet.
Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing a
container of isopentane in a container of Liquid Nitrogen
I just wanted to clarify that we don't use Novec 7000 for freezing of
muscle tissue. We use it for other routine frozen sections on
humans.Sorry if that caused any confusion. Thanks Tim for pointing that
out!
Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
Hello HistoNetters,
I have been having a discussion with my boss about whether or not to store
the isopentane (used for freezing muscle biopsies) in the refrigerator. (An
expensive, explosion proof one that I would have to buy.)
Her only experience is with the isopentane stored in the
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, April 16, 2013 1:35 PM
To: HistoNet
Subject: [Histonet] Isopentane storage-the polling is open
Hello HistoNetters,
I have been having a discussion with my boss about whether or not to store the
isopentane (used
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, April 16, 2013 12:35 PM
To: HistoNet
Subject: [Histonet] Isopentane storage-the polling is open
Hello HistoNetters,
I have been having a discussion
-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, April 16, 2013 1:35 PM
To: HistoNet
Subject: [Histonet] Isopentane storage-the polling is open
Hello HistoNetters,
I have been having
16, 2013 2:32 PM
To: Paula Sicurello ; HistoNet
Subject: RE: [Histonet] Isopentane storage-the polling is open
Paula, we keep ours at room temp in a small flam cab near the muscle bench.
We keep two gallons on hand.
In Dapson Dapson's book Hazardous Materials in the Histopathology
Laboratory
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana
Settembre
Sent: Wednesday, 17 December 2008 5:27 AM
To: carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane
Hello Carmen,
Sorry I have
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: Markus F. Meyenhofer [mailto:mi...@superlink.net]
Sent: Wednesday, 17 December 2008 1:47 PM
To: Tony Henwood; Dana Settembre; carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane
To: antho...@chw.edu.au; carmen_loise...@hotmail.com;
histo...@pathology.swmed.edu; sette...@umdnj.edu
Subject: RE: [Histonet] Isopentane
In my experience, liquid nitrogen makes a lot of freezing artifact in
muscle tissue. I have found no better substitue for isopentane if you
want quality morphology
I have found no better substitue for isopentane
ditto with isopentane (methylbutane) here... -160 in liquid form...
though it will begin to freeze solid after several minutes...
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Histonet@lists.utsouthwestern.edu
Hello ,
I currently work in a neuromuscular lab and all muscle biopsies received should
be cut frozen, no formalin fixed tissues. My concern though, is to work with
isopentane , a well known excellent heat conductor but also it has the bad
reputation of being very explosive. In fact , I've
Hello Carmen,
Sorry I have no alternative. I know most labs use that.
When I worked in a neuropathology lab the isopentane was kept in the
refrigerator - an explosion proof one.
I believe that means there's no lightbulb inside and no inner working
of a lightbulb inside either.
Dana Settembre,
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