I would like to thank all of you for your advice --
Using Thiosemicarbazide after the periodic acid did the trick, along with
making up the silver solution immediately before use. It only took ~30 minutes
in the warm silver solution before the basement membranes reached the desired
staining
Hi Jordan,
So wow! You are doing a Jones with an H
What you are seeing is common when using a water bath and preheating. While
you can perform the stain with this many sections, I would start off with 1-3
slides until you have perfected the technique.
I know that makes you have multiple runs,
Make sure the periodic acid is made fresh EACH time you run the stain.
That can also make a big difference in the stain quality.
Colleen Forster HT(ASCP)QIHC
On Thu, Sep 23, 2021 at 6:14 PM Tony Henwood (SCHN) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> I agree with Bryan,
>
>
I agree with Bryan,
The introduction of thiosemicarbazide before the silver step improves the
staining immensely.
I would also look at the periodic acid. Is it too dilute, though 0.5% should
work? I usually cover this by using a 1% solution for 20 minutes.
Regards
Tony Henwood JP, MSc,
Hi,
Try the method given in StainsFile at:
http://stainsfile.info/stain/metallic/jones.htm
Bryan Llewellyn
Hood, Jordan via Histonet wrote:
Hello,
I'm new to histology (and new to histonet), and I work in a small histology lab
specializing in animal tissues that receives
Hello,
I'm new to histology (and new to histonet), and I work in a small histology lab
specializing in animal tissues that receives requests/submissions from
researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain
on a client's submission of pig kidneys (formalin-fixed,
