Dear All I wonder if some of you could answer the following for my colleagues and myself.
For many years we have traditionally used our own 'in house' prepared Lactophenol Cotton Blue, with or without KOH as an initial screening tool. The question I have is which strength of Lactic Acid are others using if they still make their own formulation. We have always used 80% puriss grade. Our formulation is. Lactic Acid 80% 20ml Phenol Crystals 20g Glycerol 40ml Distilled H2O 20ml Aniline Blue 50mg The Aniline Blue is dissolved in the distilled water in a 100ml conical flask and placed on a non heated magnetic stirrer for 24 hours. The following day this solution is filtered into another flask, placed in a fume hood, then the lactic acid, phenol and glycerin are added, placed back on the stirrer for a couple of hours before being placed in the stock bottle for use. I have looked through much of the historical reference material and more recent references but nowhere it seems does anyone state the concentration of Lactic Acid. I would very much like to hear from others on their experiences of using this simple preparation and any other concentrations of Lactic Acid they have knowledge of. Kind regards Alan Taylor BSc(Hons) FRMS Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet