Hello Listers,
Does anyone know why the recommended processing for the Peloris is 47o C?
I was taught (back in the dark ages) that high temperatures during
processing were not good for the tissue. With the exception of the
paraffins, the processing temperatures were usually around 27 - 37o C.
Hello all,
I am new to your listserve. I am wondering if anyone has experience with the
Leica Peloris processor. Specifically, has anyone noticed if the higher
processing temperatures has affected any IHC staining results or FISH results.
Looking forward to responses,
Joe W. Walker, Jr.
In my experience, no. Our current IHC/ISH is as good, if not better than what
we were getting with our former processors.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
We have 4 peloris processors, run many IHC and FISH stains, none of them
have had any negative effects due to the increased temperatures. The
increased temps aren't that high, 45 degrees for all the liquids (we use
xylene) and 65 degrees for the paraffins.
Good luck
Patrick
On Fri, Apr 20,
reference.
That is how I did all my validations even when I was developing new processing
protocols.
René J.
--- On Thu, 3/29/12, Michelle Wahlsten mwahls...@yahoo.com wrote:
From: Michelle Wahlsten mwahls...@yahoo.com
Subject: [Histonet] Leica Peloris II processor validation
To: Histonet
Hi everyone,
I'm currently in process of validating the Peloris II for our lab, my first
instrument validation so I'm a bit lost as to what specifically I need to do
and document. Other than running specimens through the specific protocols to
verify the quality of the specimen with a
I LOVE my Peloris. Both of them equally!!
On Thu, Apr 15, 2010 at 2:32 PM, Davis, Michael J
mdav...@lancastergeneral.org wrote:
Moving into a new lab and have tested several processors. Need some info on
the peloris. Good and Bad. We demoed the machine for about 2 months and had
an issue
...@gmail.com
Subject: Re: [Histonet] Leica Peloris
To: Davis, Michael J mdav...@lancastergeneral.org
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Friday, April 16, 2010, 2:18 AM
I LOVE my Peloris. Both of them equally!!
On Thu, Apr 15, 2010 at 2:32 PM, Davis, Michael J
...@gmail.com
Subject: Re: [Histonet] Leica Peloris
To: Davis, Michael J mdav...@lancastergeneral.org
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Friday, April 16, 2010, 2:18 AM
I LOVE my Peloris. Both of them equally!!
On Thu, Apr 15, 2010 at 2:32 PM, Davis, Michael
We love all three of our pelori (sp?) We plan on buying another one in the
next year.
On Thu, Apr 15, 2010 at 11:32 AM, Davis, Michael J
mdav...@lancastergeneral.org wrote:
Moving into a new lab and have tested several processors. Need some info
on the peloris. Good and Bad. We demoed the
Moving into a new lab and have tested several processors. Need some info on
the peloris. Good and Bad. We demoed the machine for about 2 months and had
an issue with our biopsy tissue staining too light (HE). We could not get
this problem resolved - we were running our tissue thru the
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis,
Michael J
Sent: Thursday, April 15, 2010 1:33 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Leica Peloris
Moving into a new lab and have tested several processors. Need some
info on the peloris
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Thursday, April 15, 2010 11:58 AM
To: Davis, Michael J; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Leica Peloris
We use the Peloris
Hello-I would appreciate input and comments from anyone using the Leica Peloris
or the ASP 300, Thanks!
Regards,
Kate
Kathleen Caleri, BS, MLT, HT (ASCP)
Histology Lab Supervisor
Pathology
Roswell Park Cancer Institute
(716) 845-1329
Weeds are flowers too, once you get to know them.
We are thinking about purchasing a new tissue processor. We have looked
extensively into microwave tissue processors instead of the conventional tissue
processors we are all used to.
Recently two of my techs came back from a symposium and brought me literature
on the Leica Peloris. The
@lists.utsouthwestern.edu'
Subject: [Histonet] Leica Peloris versus Microwave Processing
We are thinking about purchasing a new tissue processor. We have looked
extensively into microwave tissue processors instead of the conventional
tissue processors we are all used to.
Recently two of my techs came back from
...@lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Tuesday, May 19, 2009 8:25 AM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Leica Peloris versus Microwave Processing
We are thinking about purchasing a new tissue processor. We have looked
extensively into microwave
No issues using the Milestone products.
Donna
Sent via BlackBerry by ATT
-Original Message-
From: Carol Bryant cb...@lexclin.com
Date: Tue, 19 May 2009 09:35:54
To: Vickroy, Jimvickroy@mhsil.com; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Leica Peloris versus
Willis
Sent: Tuesday, May 19, 2009 9:55 AM
To: Carol Bryant; histonet-boun...@lists.utsouthwestern.edu; Mr. Jim
Vickroy; Histonet
Cc: Dave Sanford; Jes Strong
Subject: Re: [Histonet] Leica Peloris versus Microwave Processing
No issues using the Milestone products.
Donna
Sent via BlackBerry by ATT
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