Hi histonet, I am back with a problem I've been having with my IHC slides. We had a PM recently and had thought that would solve this problem, but as I do more and more slides, the problem remains. I am seeing marginated staining at the edge of the tissue on the controls as well as the patient tissue. This occurs at the top, middle, and bottom of the slides. This occurs with any antibody. This does not occur with every run, but the more slides I have on the stainer, the more frequently it occurs. I have contacted Thermo, who now takes care of our old R.A.S. Microm HMS 710i, but I have further faith that the histonet can help me. I've seen similar posts in the archives, but did not see any responses.
Any suggestions are appreciated, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet