So...I have never done this before. I looked it up in the good ol' bible and
found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure
because he thought it would be more gentle. So...the slides have been sitting
in the 10% solution for 24 hours now. While it is
Hi Janci,
We have used 10% H2O2 overnight and 3% at 55 degrees for 2 hours prior to
immunostaining and both worked adequately to bleach the melanin. However, we
did have trouble with tissue falling off at 2 hours, so you might need to test
it in your own lab and see if you can get away with
Bleaching (Janci Wellborn)
2. Re: Melanin Bleaching (Rene J Buesa)
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Message: 1
Date: Wed, 26 Dec 2012 15:39:40 -0600
From: Janci Wellborn jwellb...@bjc.org
Subject: [Histonet] Melanin Bleaching
To: histonet
I am looking for alternatives to the potassium permanganate melanin bleaching
procedure when doing IHC. I have seen literature references to doing a 3% H2O2
at 55 C after the IHC staining. I have also done a Giemsa counterstain in the
past. Does any one have either procedure that they would be
, 2012 4:39 PM
Subject: [Histonet] Melanin Bleaching
I am looking for alternatives to the potassium permanganate melanin bleaching
procedure when doing IHC. I have seen literature references to doing a 3% H2O2
at 55 C after the IHC staining. I have also done a Giemsa counterstain in the
past. Does
