Amy, I can't find the exact reference but I think either Balch or Taylor authored the paper that was adapted for our protocol.
Level 1, 3, 5, 10 are H/E and extra section at level 5 is taken for Negative serum control Level 2 is S100 Level 4, 6 is PanMelanoma cocktail (Biocare Medical) Level 7, 8, 9, are held for as unstained and filed away additional H/E or IHC We usually aim for about 200 microns in between levels. This is entirely dependent on the degree of adipose tissue that was included around the node and the thickness of the node in the paraffin block. When we started this protocol about 5 years ago, we would get 1 or 2 paraffin blocks for analysis. Nowadays we typically receive 3-6 blocks per case (sometimes 2 cases per week) and this has impacted our workload. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 From: "Amy Farnan" <farn...@nehealth.com> Subject: [Histonet] sentinel lymph node melanoma protocols To: <histonet@lists.utsouthwestern.edu> Message-ID: <4de60e2b.26ed.00d...@nehealth.com> Content-Type: text/plain; charset=US-ASCII Good morning everyone, I am curious to see what the standard protocol is on sentinel lymph node for melanomas. I need to establish a protocol at my institution and I have been reading a lot of literature and it seems to be a wide variety of thinking out there from bread loafing the lymph node first then cutting multiple H&E sections and IHC in between to bivalving. Some of the protocols call for up to as many as 46 H&E sections at 3 microns but it doesn't say where to take the sections for the IHC (S100, pan-melan cocktail). Would any of you like to share your protocols? thank you- Amy Farnan Histology Supervisor Northeast Health Albany, New York Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ------------------------------ Message: 7 Date: Wed, 1 Jun 2011 09:40:03 -0700 (PDT) From: Dawud AlYasa <dawudh...@yahoo.com> Subject: [Histonet] Bone Marrow H&E Protocols To: histonet@lists.utsouthwestern.edu Message-ID: <759774.67432...@web34506.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histonet! ? Does anyone have an H&E automated stainer protocol for bone marrows that they wouldn't mind sharing? I'm looking at different protocols. It would be greatly appreciated. Thanks. ? Dawud ------------------------------ Message: 8 Date: Wed, 1 Jun 2011 09:54:01 -0700 From: "Grantham, Andrea L - (algranth)" <algra...@email.arizona.edu> Subject: [Histonet] pic of tattooed skin To: HISTONET <histonet@lists.utsouthwestern.edu> Message-ID: <55dcef43-7dd7-4117-a62d-5790da604...@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" Good Morning! Found out a few days ago that next week we are participating in a summer camp for high schoolers and it was decided to use skin this year for our examples. I have lots of slides of skin sections from mice, rats, frogs, etc. and even some human skin punches - normal and with skin cancers from when we did a project for a sunscreen manufacturer. What I was looking for was an example of tattooed skin and I spent a bit of time searching for one yesterday online but couldn't find anything that was suitable. Does anyone out there have a picture of skin that has been tattooed? And maybe a good skin scar? Last year's camp was on liver, which was way easier! Thanks. Andi Grantham ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 91, Issue 1 *************************************** Legal Disclaimer: Information contained in this e-mail, including any files transmitted with it, may contain confidential medical or business information intended only for use by the intended recipient(s). Any unauthorized disclosure, use, copying, distribution or taking of any action based on the contents of this email is strictly prohibited. Review by any individual other than the intended recipient does not waive or surrender the physician-patient privilege or any other legal rights. If you received this e-mail in error, please delete it immediately and notify the sender by return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet