[mailto:darkd...@comcast.net]
Sent: Wednesday, March 27, 2013 5:26 PM
To: Johnston, Amy
Subject: Re: [Histonet] Methanol
Amy,
Are you aware that Formalin Fixative contains Methanol, usually a little over
1%? The label and MSDS should state this.
Mark
On 3/27/2013 6:47 PM, Johnston, Amy wrote:
Hello all
: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date: 04/02/2013 02:45 PM
Subject:RE: [Histonet] Methanol
Sent by:histonet-boun...@lists.utsouthwestern.edu
Thank you,
But they weren't concerned with the methanol in the formalin, it was the
methanol
I would contact OSHA and tell them the specifics. The possible vapor
contribution of 4 ml of methanol, if it is even measurable, is certainly
insignificant from a health/safety perspective.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hello all,
I work in a medical lab that has recently expanded to histology and we are
working on our chemical hygiene plan. We have already done the initial air
sample testing for formaldehyde and xylene, but we were told by someone at OSHA
that we need to test for methanol as well. The only
Hello all
If tissue (from autopsy cases) has been fixed in 10% formal saline,
will there be a difference in the stained section if:
processed in methanol
processed in ethanol
Thanks
Michelle
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic
AM
Subject: [Histonet] methanol vs ethanol in processing
Hello all
If tissue (from autopsy cases) has been fixed in 10% formal saline,
will there be a difference in theĀ stained section if:
processed in methanol
processed in ethanol
Thanks
Michelle
Michelle Perrins
Chief Medical Technologist
Hi Histonetters,
This has been a fun conversation. I called up a technical support scientist at
Vector Labs with the question and was told basically let me get back to you on
that. :)
But with some digging and the reference from Gudrun (thanks!) I've pieced
together the following
Hi everyone,
Can someone refresh/enlighten me as to why Methanol is used in H2O2 blocking.
Let's assume that I take my slides through the xylene/ethanol steps and that
after the 70% etoh wash step, they are now in TBST.
I could see adding methanol if you were doing the H2O2 blocking as part of