If you choose another oxidizing agent than CrO3 (such as KMnO4, potassium
permanganate) it's up to you either to find literature to back you up, or
to do the stain with appropriate controls for the fungus you're looking for.
Freida Carson (citation below) found that periodic acid, commonly
substit
r a sufficient result.
> (5 min in periodic acid may refer to 20 sek in potassiumpermanganat ?)
>
> Gudrun
>
>
>
> -Ursprüngliche Nachricht-
> Von: Nipuna Weerasinghe via Histonet [mailto:histonet@lists.
> utsouthwestern.edu]
> Gesendet: Donnerstag, 28. Dezember 2017 20:30
>
potassiumpermanganat ?)
Gudrun
-Ursprüngliche Nachricht-
Von: Nipuna Weerasinghe via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 28. Dezember 2017 20:30
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Modified GMS Protocols
I would like to know from subje
I would like to know from subject matter expert why KMno4 never used as an
alternative oxidant to CrO3 in Modified GMS. Oxidation of 1,2-glycol
linkages in carbohydrates to aldehyde groups can be done by KMNO4 and used
to do the same in Castella’s potassium permanganate-Schiff reaction, and
Gordon
, November 19, 2010 11:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Modified GMS
Hi all. Years ago, I used a modified GMS procedure that used periodic acid
in
place of chromic acid. Would anyone be willing to share their procedure for
this
or at least a vendor you might be
We purchase a kit from Sigma, cat #HT-100A-1KT.
Message: 14
Date: Fri, 19 Nov 2010 08:16:35 -0800 (PST)
From: Sheila Haas
Subject: [Histonet] Modified GMS
To: histonet@lists.utsouthwestern.edu
Message-ID: <24062.7620...@web57806.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-
Hi all. Years ago, I used a modified GMS procedure that used periodic acid in
place of chromic acid. Would anyone be willing to share their procedure for
this
or at least a vendor you might be purchasing a kit from? Thanks in advance!
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories,
