RE: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67

2014-10-08 Thread James Watson
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, October 08, 2014 8:52 AM To: Melanie Smith; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Melanie, We do this stain, we

RE: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67

2014-10-08 Thread James Watson
: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too

[Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67

2014-10-08 Thread Melanie Smith
Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too much autofluorescence even after trying several methods of knocking it do