[Histonet] Muscle Enzyme Histochemistry

2019-04-16 Thread MONICA D. LOCKHART via Histonet
Hello Histonet!! Hope all is well. My lab would like to replace 2 techniques used during muscle workup with Myosin Fast and Myosin Slow. Is there a lab running these tests that would give us guidance as to how to work these up? Also, we are looking to replace our IDH-1 Antibody by Dianova.

Re: [Histonet] Muscle Enzyme Histochemistry

2019-02-25 Thread Bryan Llewellyn via Histonet
Hi, I presume you are talking about light microscopy. Glutaraldehyde is often used for electron microscopy. If that is the case, ignore this. Glutataraldehyde fixes similarly to formalin, so the morphology should not be much different. However, it leaves the tissue with free aldehyde groups

[Histonet] Muscle Enzyme Histochemistry

2019-02-25 Thread MONICA D. LOCKHART via Histonet
Hello Histology World!!! Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. Thanks for your help. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical