After grossing in, we soak the nail in Nair (dab of HOT water mixed to
runny paste) for a couple of hours to soften the nail.
We face into the block, soak the face of the block in fresh Nair solution
again for at least 30 minutes.
Bake sections in the oven for 1 hour at least, sometimes hand stain
Does anyone have any special tricks to keeping nail sections on the slides? I
am doing a PAS stain and the tissue keeps falling off.
Using + Slides, cut on 1-2 microns, baking 1/2 hr. Air drying, and this nail
still wants to fall off.
Any suggestions. Please help,
Craig
Sent from my
Our lab receives toenails, and we routinely do an HE stain and a PAS stain for
fungus. We receive the nails fresh, and when they are grossed they are placed
directly into a purchased nail softening solution that is mainly potassium
hydroxide before being placed on the tissue processor. My
or else they will get washed off slides.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Friday, November 22, 2013 7:48 AM
To: Histonet@Lists. Edu
Subject: [Histonet] Nails falling off slides
Hi All,
I know this has been mentioned a few times but need some help with nails
falling off charged slides for HE and PAS stains. I use Statlab nail prep
softener and also use the good charged slides. I heat them 2 times for 25
minutes each. Is there an adhesive I can put on slide? Albumin?
Of course this is only great if you have a day to spare. :)
From: lcolb...@pathmdlabs.com
To: histonet@lists.utsouthwestern.edu
Date: Mon, 29 Oct 2012 18:09:46 +
Subject: [Histonet] Nails
Is there anyone out there who is an expert on nails - specifically how to
get them to stay on the slides
Is there anyone out there who is an expert on nails - specifically how to get
them to stay on the slides?? We use Nair to soften the nails, and we have used
plain plus slides, chrome alum, Elmer's glue, and gelatin - all with very
limited success.
Laurie Colbert, HT (ASCP)
Histology
This helps to keep sections of bone from floating. I mount them on slides
coated with water resistant wood glue, then immediately microwave dry the
slides. 40% power for 3 minutes. No doubt there will be variations, depending
on the oven.
Laurie Colbert lcolb...@pathmdlabs.com 10/29/2012
: [Histonet] Nails
Is there anyone out there who is an expert on nails - specifically how to get
them to stay on the slides?? We use Nair to soften the nails, and we have used
plain plus slides, chrome alum, Elmer's glue, and gelatin - all with very
limited success.
Laurie Colbert, HT (ASCP
Hi Everyone in Histoland,
Having problems keeping my nails on plus slides when staining. They of
course fall off during the PAS staining process. Can anyone help me
please, thank you! Kristy
___
Histonet mailing list
I use a 5% solution of Titebond 2 wood glue to coat slides. You should be able
to find it at your local hardware store. Works well for me on bone and bloody
tissue, Occasionally I need to microwave dry slides of very dense bone (skull,
inner ear).
kcasti...@frii.com 8/23/2012 10:29 AM
Hi
Hello to everyone. What is the general concensus on softening up of nails
before cutting? Does anyone have a process that the gross tech follows or is
it done at the microtome bench?
Histopathy
___
Should be softened BEFORE tissue processing using 10% aq. sol. of sodium
hydroxide during 30 min → wash → process as usual.PRené J.
--- On Sun, 11/28/10, Pamela Gholston histopa...@hotmail.com wrote:
From: Pamela Gholston histopa...@hotmail.com
Subject: [Histonet] Nails softened up during
...@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Friday, November 19, 2010 9:51 AM
To: 'Shirley A. Powell'; godsgal...@aol.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nails
An old old trick is taking a plain slide and coating it with dilute Elmer's
glue and letting it dry. Be quick
The least complicated way to cut nails is to face the block, get a small
shallow dish and put in a few drops of Dawn dish soap and add a little warm
water. Just soak for about 10 minutes. Cut and place on a + slide and dry
slide well before staining.
We are having a very difficault time with the naisl falling off the slides when
we do the PASF stain. We are using charged slides already and we have tried
increasing the baking time during drying to no avail.
Any suggestions?
Roxanne
___
...@aol.com
To: histonet@lists.utsouthwestern.edu
Sent: Fri, November 19, 2010 11:27:49 AM
Subject: [Histonet] Nails
We are having a very difficault time with the naisl falling off the slides when
we do the PASF stain. We are using charged slides already and we have tried
increasing the baking time
] On Behalf Of
godsgal...@aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails
We are having a very difficault time with the naisl falling off the slides when
we do the PASF stain. We are using charged slides already and we have tried
] On Behalf Of Shirley A.
Powell
Sent: Friday, November 19, 2010 11:40 AM
To: godsgal...@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Nails
Switch to Stay On and plain slides, I had problems with charged slides and now
have good results with the additive and regular slides. Cheaper
, November 19, 2010 8:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails
We are having a very difficault time with the naisl falling off the slides when
we do the PASF stain. We are using charged slides already and we have tried
increasing the baking time during drying to no avail
, Judith [mailto:spath.jud...@marshfieldclinic.org]
Sent: Friday, November 19, 2010 11:49 AM
To: powell...@mercer.edu
Subject: Re: [Histonet] Nails
Right now, there is a serious BACK ORDER on STA ON...just an FYI
--Original Message--
From: Shirley A. Powell powell...@mercer.edu
Date
We are having problems getting nail sections to stick to the slide. They
almost always fall off the slide during HE staining. We use plus slides and
various adhesives - sta-on, hairspray, etc. with no success.
Is there anybody that can help me by sharing their secret method to get nail
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