What we do here is run a mix of expected negative and positive cases this way
we are sure we are not getting exogenous staining. Depending on the antibody
we try to run at least a mix of 25 cases. In some cases the antibody proves
difficult to get a large amount of positive cases and we make
We are beginning the process of validating new antibodies using the BenchMark
XT but have some questions for validation.
Question: For a positive reagent control that stains appropriately positive,
is a known control block used? If not, what is used?
Question: For a negative reagent control th
