Hello,
We tried to do PAS staining on kidney. I attached my picture here. I am
looking for the staining results that has more reddish as showing in the
second picture. So I can do quantification better. We purchased Schiff's
reagent from Sigma freshly. Could anybody teach us how can I can the
I have for some time had a problem with Schiff's reagent and PAS staining.
- I have tested each new, unopened bottle of Schiff's reagent with
formaldehyde and always the color development was immediate, but purple,
definately not pink.This result has been quite consistant.
- PAS
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janet Keeping
Sent: Wednesday, October 06, 2010 11:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS staining
I have for some time had a problem with Schiff's reagent and PAS staining.
- I have
Although I have allowed Schiff's and Periodic acid to room temperature,
temperature may indeed be a factor. The ventilation system within this lab
is extremely effficient and takes in a great deal of outside air. I am in
Newfoundland, and lower outside temperatures are more common than we
:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS staining
I have for some time had a problem with Schiff's reagent and PAS staining.
- I have tested each new, unopened bottle of Schiff's reagent with
formaldehyde and always the color development was immediate, but purple
To: 'Janet Keeping'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] PAS staining
Yes, I have experienced the same thing. Many people forget about the impact
temperature has on some staining reactions. If you keep your Schiffs and
Periodic acid in the refrigerator it may take longer to stain
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janet
Keeping
Sent: Wednesday, October 06, 2010 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS staining
I have for some time had a problem with Schiff's reagent and PAS staining.
- I
06, 2010 11:58 AM
To: 'Mahoney,Janice A'; 'Janet Keeping'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] PAS staining
Jan is correct, and unless specified in a staining protocol, room
temperature is used.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janet
Keeping
Sent: Wednesday, October 06, 2010 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS staining
I have for some time had a problem with Schiff's reagent and PAS staining.
- I
I still make my own Schiff's reagent. It is easy to make and I've
never had a problem with it. Keeps well in refrigerator. I do make the
periodic acid fresh each time. If anyone wants the recipe, let me know.
LuAnn
On 10/6/2010 11:43 AM, Janet Keeping wrote:
I have for some time had a
October 2010 3:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS staining
I have for some time had a problem with Schiff's reagent and PAS
staining.
- I have tested each new, unopened bottle of Schiff's reagent with
formaldehyde and always the color development was immediate
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