-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks
Sent: Wednesday, February 09, 2011 7:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS with Diastase Digestion
Hi Histonet-
I recently ran a PAS/D stain and had some
du
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks
Sent: Wednesday, February 09, 2011 7:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS with Diastase Digestion
Hi Histonet-
I recently ran a PAS/D stain and had some issues with it. Both "with" and
&q
You CAN NOT use water to dissolve the diastase. You have to use diastase buffer
pH7 otherwise it will not work (as in your case).
René J.
--- On Wed, 2/9/11, Erin Sarricks wrote:
From: Erin Sarricks
Subject: [Histonet] PAS with Diastase Digestion
To: histonet@lists.utsouthwestern.edu
Date
...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks
Sent: Wednesday, February 09, 2011 7:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS with Diastase Digestion
Hi Histonet-
I recently ran a PAS/D stain and had some issues with it. Both "with" and
"without" sli
Hi Histonet-
I recently ran a PAS/D stain and had some issues with it. Both "with" and
"without" slides came out looking the same so I'm guessing my digestion step
didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my
digestion. This is the procedure I used:
1.Depar