RE: [Histonet] PAS with Diastase Digestion

2011-02-10 Thread Susan.Walzer
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some

RE: [Histonet] PAS with Diastase Digestion

2011-02-09 Thread Monfils, Paul
du [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and &q

Re: [Histonet] PAS with Diastase Digestion

2011-02-09 Thread Rene J Buesa
You CAN NOT use water to dissolve the diastase. You have to use diastase buffer pH7 otherwise it will not work (as in your case). René J. --- On Wed, 2/9/11, Erin Sarricks wrote: From: Erin Sarricks Subject: [Histonet] PAS with Diastase Digestion To: histonet@lists.utsouthwestern.edu Date

RE: [Histonet] PAS with Diastase Digestion

2011-02-09 Thread Tom McNemar
...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and "without" sli

[Histonet] PAS with Diastase Digestion

2011-02-09 Thread Erin Sarricks
Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both "with" and "without" slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1.Depar