We use Bond slides and have noticed that occasionally they seem to have an
extra thick coating on them that will pick up DAB and turn slides brown. If
the glass itself is turning brown this might be your issue. Have you tried
different slide lots or brands?
Cathy Crumpton HT(ASCP), Lead
Anybody else having problems with PIN 4 staining (Biocare)? We are getting
brown background staining, even on the glass itself where there is no tissue.
After many years of fantastic results, this problem has occurred recently. Our
AMACR is also a lot fainter than it used to be. We've switched
