Re: [Histonet] Pas Digestion

2015-11-04 Thread Caroline Miller via Histonet
s in 37 degrees Celsius. > > > > > From: "Lager, Loree via Histonet" > To: "'histonet@lists.utsouthwestern.edu'" > > Date: 11/03/2015 12:38 PM > Subject:[Histonet] Pas Digestion > > > > Hello, > > This is my

Re: [Histonet] Pas Digestion

2015-11-03 Thread Ray via Histonet
uot; Sent: Tuesday, November 3, 2015 12:37:04 PM Subject: [Histonet] Pas Digestion Hello, This is my first time using the histonet, as our veteran user retired. We've been having an intermittent problem with incomplete glycogen digestion on PASD.  Our digestion solution is 0.5%  α-Amylas

Re: [Histonet] Pas Digestion

2015-11-03 Thread Jennifer MacDonald via Histonet
The optimal temperature for most enzymes in 37 degrees Celsius. From: "Lager, Loree via Histonet" To: "'histonet@lists.utsouthwestern.edu'" Date: 11/03/2015 12:38 PM Subject: [Histonet] Pas Digestion Hello, This is my first time using the

[Histonet] Pas Digestion

2015-11-03 Thread Lager, Loree via Histonet
Hello, This is my first time using the histonet, as our veteran user retired. We've been having an intermittent problem with incomplete glycogen digestion on PASD. Our digestion solution is 0.5% α-Amylase, Type VI-B from porcine pancreas (Sigma), which we freeze in small aliquots and thaw for