Re: [Histonet] Phospho-Histone 3 (SER10) antibody

2010-07-14 Thread Hobbs, Carl
I use Upstate's 06-570. Excellent for human, rat, mouse FFPW sections after HIER. I have no idea what amino acid is phosphorylated but, if you are wanting an Ab against cells in mitosis only, this is the best, imho. Carl Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases

RE: [Histonet] Phospho-Histone 3 (SER10) antibody

2010-07-14 Thread Connolly, Brett M
brett_conno...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Wednesday, July 14, 2010 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Phospho-Histone 3

[Histonet] Phospho-Histone 3 (SER10) antibody

2010-07-12 Thread Martha Ward
I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated.

RE: [Histonet] Phospho-Histone 3 (SER10) antibody

2010-07-12 Thread Connolly, Brett M
...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Monday, July 12, 2010 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Phospho-Histone 3 (SER10) antibody I have been