Hi,
Does anyone have a good enzyme method for Phosphorylase done on muscle
bx's. We have been doing the method from Dubowitz, 2nd edition for years
but would like to get away from the messy PVP coverslipping. We are
trying cytoseal, which seems to work fine for coverslipping, but would
like to
Jean A [mailto:jmitch...@uwhealth.org]
Sent: Wednesday, March 28, 2012 9:07 AM
To: Sharon Allen
Subject: RE: [Histonet] Phosphorylase method
Sharon: Just curious - Does the phosphorylase method you use fade after
time?
The method I use does use PVP and dextran (no glyceron though). I am
: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Phosphorylase method
Thanks for the quick response.
I would love to take a look at your method. Our method from Dubowitz's
2nd edition has worked fine for years, but can leave residue on the
slides, especially when fresh, fades is very
