Dear Yoanna I performed Picrosirius Red staining on my tissue frozen
sections with good results so I suggest you operate as follows:
1)Fix slide in 4% PFA (5 min);
2)Rinse in ddH2O;
3)Incubate in Phosphomolybdic Acid (2 minutes);
4)Rinse in ddH2O;
5)Incubate in Picrosirius Red for (1 h);
6)Wash
: Mon, 11 Nov 2013 19:34:32 -0500
From: Lee Peggy Wenk lpw...@sbcglobal.net
Subject: Re: [Histonet] PicroSirius Red in Frozen Sections
To: Yoanna Bello Arredondo bellopa...@yahoo.es,
Histonet@lists.utsouthwestern.edu
Message-ID: 2ABC0BE3B30C4B8091C82B0CC2CA35F1@HP2010
Content-Type: text
Hello!
I did a Picrosirius red stain using the polysciences kit in paraffin section
cut at 4microns. The stain looks great. Later on my boss asked me to do the
picro using the same kit but in frozen section. The stain looks very, very
intense. Sections are really red. When looking under the
] PicroSirius Red in Frozen Sections
Hello!
I did a Picrosirius red stain using the polysciences kit in paraffin section
cut at 4microns. The stain looks great. Later on my boss asked me to do the
picro using the same kit but in frozen section. The stain looks very, very
intense. Sections are really
