RE: [Histonet] Picrosirius red on cryosections

2013-04-16 Thread Elizabeth Chlipala
, 2013 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Picrosirius red on cryosections Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound

[Histonet] Picrosirius red on cryosections

2013-04-16 Thread Laura Avogaro
Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative. Does anyone can suggest me a protocol to perform this staining? Thanks in advance