, mostly with snap freeze
or meoh/acetone fixed tissues without cryoprotectant.
Hope this helps.
-Mehlika
> Date: Fri, 7 Oct 2011 14:36:24 +1100
> From: julie.bil...@gmail.com
> To: pbo...@histoprep.com
> Subject: Re: [Histonet] Poor quality frozen sections, feline skin.
&g
Hi Peter,
We use 20-25% sucrose on our fresh specimens for immunofluorescence (IF). We
only deal with human skin samples so I will not comment on feline skin. I
sit the specimen in three changes of sucrose for 10 minutes each. Before
embedding in the cryostat, I gently blot it on a tissue. My unde
I have some feline skin samples for frozen sections that were sent to us
because our client was not able to section them themselves. Unfortunately we
are having the same problems trying to make good sections ourselves. The
samples seem dry and unsupported when we try to cut them. The tissue brea
