[Histonet] Primary works with ABC but not IF

Hello- I am in a situation where my primary antibody works using the ABC method, but I do not see staining with immunofluorescence. My IF protocol works well with other antibodies so I do not know why it's not working with this particular antibody. Does anybody have any suggestions? I have

Re: [Histonet] Primary works with ABC but not IF

: Patten, Nicole (NIH/NIAAA) [F] [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 10, 2008 10:09:45 AM Subject: [Histonet] Primary works with ABC but not IF Hello- I am in a situation where my primary antibody works using the ABC method, but I do not see staining

Re: [Histonet] Primary works with ABC but not IF

: Re: [Histonet] Primary works with ABC but not IF Hi Nicole, I don't know what kind of secondary antibody you are using, but we have had very good luck with the Alexa-Fluor secondaries.  Often, when you go from an amplified chromagenic method (such as ABC) to a less-amplified staining method