Hello, I am having problems staining my FFPE mouse brains for macrophages. I started out with F4/80 marker, but have now moved to CD68. My positive controls (mouse spleen and liver) work fine, but I cannot get anything to stain in my sample tissues. I have see through H and E staining that macrophages are present, could anyone help me with this problem? Mike Valerio University of Buffalo
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