Hi, I am new to Histonet. Here is my question.
We have brain samples that were fixed in 4% paraformaldehyde then placed in 30% sucrose (cryoprotectant) prior to freezing on dry ice. These samples have been stored at -80 for a couple of years. We want to thaw these samples then process them to make paraffin embedded blocks. What is the best way to remove the sucrose prior to processing? Should we just rinse with PBS? Thank you, Valerie Valerie Ratliff B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murp...@karmanos.org<mailto:murp...@karmanos.org> Better treatments. Better outcomes. ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
