Well, I have a number of suggestions now; once I get past the holidays
I'll start trying them out when the next batch of samples comes in. We
provide histology and pathology services for our dept and university, so
it's all animal tissue.
Thanks again to everybody, and I'll let you know how it turns out.
-Kathleen
Rutgers University
Rene J Buesa wrote:
Mouse tissues, any one, will dry out too much with ethanol and xylene.
I advise you to stop using ethanol and xylene and substitute BOTH with
iso-propanol at the concentrations and times you are using now.
Instead of the first paraffin, use a mixture 1:1 of iso-propanol and
paraffin, followed by the 2 paraffins.
Try it and you will notice the difference (and the saings).
René J.
--- On Wed, 12/10/08, Kathleen Roberts <[EMAIL PROTECTED]> wrote:
From: Kathleen Roberts <[EMAIL PROTECTED]>
Subject: [Histonet] Processing mouse seminal vesicles
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Date: Wednesday, December 10, 2008, 11:54 AM
Does anybody have a protocol for this? My last batch of these came out VERY dry
and crunchy when run with other tissues on my standard protocol, which is as
follows:
(They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out before
processing.)
70%: 30 min
80%: 30 min
95%: 45 min
95%: 45 min
100%: 45 min
100%: 45 min
xylene: 45 min
xylene: 45 min
Paraffin: 30 min
Paraffin: 30min
Paraffin: 30 min
My other thought is that something is up with our VIP 5 processor, though no
error messages are showing up. Any and all suggestions are most welcome.
Thanks in advance,
Kathleen Roberts
Rutgers University
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