-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Hello all,
<<>>>
We use start at 95%, but we have two Pen Fix (alcohol fixative) steps between
our Formalin and alcohols. 10% NBF, 10% NBF, Pe
Hi Tom:
I deal exclusively with post-mortum brain tissue, so my situation may
not apply to you.
I do not use formalin on my processor, since the half brain used for
brain-cutting has already been thoroughly fixed.
So, I have the luxury of using 30%, 50%, 80%, 95%, then three 100%
Isopropanols.
;
Sent: Thursday, February 7, 2013 10:14 AM
Subject: [Histonet] Processor dehydration cycles..
Hello all,
I was wondering what most people use as the first reagent after the formalins
on their tissue processor? We have always used a sequence of 70%, 80%, 95%,
and 100% but is anyone using
Hello all,
I was wondering what most people use as the first reagent after the formalins
on their tissue processor? We have always used a sequence of 70%, 80%, 95%,
and 100% but is anyone using 80% or even 95% to start their dehydration?
Thanks in advance.
Tom McNemar, HT(ASCP)
Histology Co-o
