Hi Christina:
I use Paraplast to infiltrate the formalin fixed brain tissue, but I use
Surgipath Embedding Medium from Leica (Catalogue number 3801320) to
embed the tissue.
(Surgipath does have about the same melting point as the Paraplast)
Even the 10 micron sections (for the Congo red stain
Hi,
Can anyone with experience in cutting thick FFPE brain sections please give
advice on how to get a thick brain section without it curling up before it gets
to the waterbath? We are doing okay at cutting these at 10 microns, but are
trying to get some 12 micron sections and the section just