: Re: [Histonet] Question concerning H. pylori staining
Hi!
I found this instruction for a Hp- stain, that sounds similiar to yours.
They want the slides to be airdried after water-rinsing and before xylen.
But the result should be blue bacteria, not purple.
I would try to let the slides air-dry
with
Alcian yellow, to give a more contrasted result.
Gudrun Lang
-Ursprüngliche Nachricht-
Von: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 9. April 2020 20:05
An: Histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Question concerning H
Michelle (where) asksi: >>We have a question about staining for H-Pylori
Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,
Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like
the H-Pylori purple stained clusters, but after dehydration in 100% alcohol
Hi everyone! We have a question about staining for H-Pylori Using Quick Stain
(Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock,
Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple
stained clusters, but after dehydration in 100% alcohol the