RE: [Histonet] Question on staining

2008-09-17 Thread Jack Ratliff
rry R <[EMAIL PROTECTED]> Sent: Wednesday, September 17, 2008 11:39 AM To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question on staining There is a question on the validity of measuring osteoid on thick sections. The reason is that it is rare in bone to ha

RE: [Histonet] Question on staining

2008-09-17 Thread Rittman, Barry R
this thick? Barry -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Herrick, James L. Sent: Tuesday, September 16, 2008 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on staining Hi all, I am trying to stain for osteoid and bone

[Histonet] Question on staining

2008-09-16 Thread Herrick, James L.
Hi all, I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 µm thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well de