RE: [Histonet] RE: "Vacuole" appearance in lymph Nodes

2013-02-15 Thread Britton, Josette C
) Sent: Thursday, February 14, 2013 6:35 PM To: 'Metzger, Kenneth'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: "Vacuole" appearance in lymph Nodes Ken, Are the Microtomists being over enthusiastic with the block "trimming" or "facing"? This

[Histonet] RE: "Vacuole" appearance in lymph Nodes

2013-02-14 Thread Tony Henwood (SCHN)
Ken, Are the Microtomists being over enthusiastic with the block "trimming" or "facing"? This is more prone to happen if the block is cold (ie harder) during trimming. I would recommend after trimming to full-face that a few slower turns of the microtome wheel at 4-5 microns will clear the holes