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Sent: Thursday, February 14, 2013 6:35 PM
To: 'Metzger, Kenneth'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: "Vacuole" appearance in lymph Nodes
Ken,
Are the Microtomists being over enthusiastic with the block "trimming"
or "facing"?
This
Ken,
Are the Microtomists being over enthusiastic with the block "trimming" or
"facing"?
This is more prone to happen if the block is cold (ie harder) during trimming.
I would recommend after trimming to full-face that a few slower turns of the
microtome wheel at 4-5 microns will clear the holes