We use H.Pylori from Cell Marque and the Benchmark XT as well. When we began doing the H.Pylori by immuno (versus Giemsa) we had had the same problem. Tech support from Cell Marque suggested using a Protease 2 (from Ventana) to decrese background, and it worked. Here's our protocol:
CC1 Mild (30 min) Protease 2 (4 min) H.Pylori (32 min) Hematox (4 min) Bluing (4 min) Hope this helps!! -- Adrienne Kavanagh HTL (ASCP) US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
