Amy,
You wrote:
A wonderful colleague recommended to aid in my response that I add the
components I am using for preparation to assist in obtaining a more specific
response, about shelf life, so here it is:
0.1 M Tris/HCl Buffer pH 7.4 - to which is added:
Calcium Acetate -
:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: zinc fixative
Amy,
You wrote:
A wonderful colleague recommended to aid in my response that I add the
components I am using for preparation to assist in obtaining a more specific
response, about shelf life, so here
You wrote:
I have recently used zinc fixed tibia and calvaria for the staining of CD31.
My CD31 does seem to have worked, however there seems to be marrow loss and
digestion of the marrow also.
I zinc fixed the bones for 48 hours and then used EDTA pH 7 for
decalcification for 2
