[Histonet] RE: Antibody IDH1

2013-03-04 Thread Sue Hunter
We use Dianova's antibody at a 1:100 dilution on the Ultra. CC1 cell conditioning for 40 minutes, antibody for 32 minutes - no heat. We use Optiview detection system. No amp. Make sure you are using the correct type of brain tissue for a control - most oligodendrogliomas should be positive, a

[Histonet] RE: Antibody IDH1

2013-02-22 Thread Bitting, Angela K.
Are you using the antibody from Dianova?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Friday, February 22, 2013 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet]