We use Dianova's antibody at a 1:100 dilution on the Ultra. CC1 cell
conditioning for 40 minutes, antibody for 32 minutes - no heat. We use
Optiview detection system. No amp. Make sure you are using the correct type of
brain tissue for a control - most oligodendrogliomas should be positive, a
Are you using the antibody from Dianova??
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman,
Billie
Sent: Friday, February 22, 2013 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]