There is no one right answer for the number of cases needed to validate an
antibody for diagnostic immunohistochemistry. That decision must be made by
the laboratory's medical director, not an outside organization. Many of the
primary antibodies available today have proven track records and
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We work out the specifics of the protocol for each new antibody with variables
such as dilution, heat or enzyme retrieval, detection system and antibody
incubation times based on the manufacturer's recommendations in the insert for
the antibody. We do this
