I was very impressed with the Biocare instrument. It is called the Intellipath. Open system (my favorite feature), continuous feed up to 10 slides per load, able to run 5 protocols simultaneously for a total of 50 slides running simultaneously- can't remember how many protocols it can store. Definitely worth a look.
Diana Goodwin Anatomic Pathology Pennsylvania Hospital Philadelphia, PA. -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Tuesday, September 23, 2008 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [EMAIL PROTECTED] You can reach the person managing the list at [EMAIL PROTECTED] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Microtome micron calibration and validation (Randall Carpenter) 2. Re: Humidity levels in the lab (Robert Richmond) 3. RE: Oversized microscope supply source (IN USA) (Jerry Helisek) 4. Histology schools/training in San Antonio, TX (Jackie M O'Connor) 5. Microtome ([EMAIL PROTECTED]) 6. Seattle-Tacoma craigslist: Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) (Owen, Michael P) 7. Re: Cassette Holders (Joseph Saby) 8. BioCare (Jon Google) 9. Re: BioCare (Roxanne Soto) 10. Cryosectioning dorsal mouse skin (Orlowski Christian) 11. detection of brain infarcts (Sohail Ejaz) 12. Re: Blind Review Policy ([EMAIL PROTECTED]) 13. RE: Cassette Holders (Ford Royer) 14. Effect of 5%Aeti acid on Nuclei (Trajkovic, Dusko) 15. Re: Effect of 5%Aeti acid on Nuclei (Rene J Buesa) 16. Re: Re: Blind Review Policy ([EMAIL PROTECTED]) 17. Re: Cassette Holders ([EMAIL PROTECTED]) 18. RE: Ventana Retic Stain (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 Sep 2008 10:53:19 -0700 (GMT-07:00) From: Randall Carpenter <[EMAIL PROTECTED]> Subject: [Histonet] Re: Microtome micron calibration and validation To: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=UTF-8 I just did an IQ, OQ, PQ on my process. The OQ on the microtome involved using a dial micrometer. Set the thickness at 5 microns. Tare or zero the dial micrometer on a point on your sample holder. Pull the micrometer point back, turn the wheel X number of times and take your new reading. It should add up to X times 5 microns +/- your tolerances. The rest of the "validation" process depends on it being installed properly and that it actually cuts sections suitable for pathologic interpretation. As we all know, just because the dial on the microtome says 5, doesn't mean the section is 5 microns. The point of the Operation Qualification is only to demonstrate that the machine works like it's supposed to. The dial micrometer needs to be calibrated/traceable. I got a guy in town to do it for $20. Of course everything needs to be documented and QA should sign off on it. Hope it helps. Randy Carpenter Twin Cities Histology Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research ------------------------------ Message: 2 Date: Mon, 22 Sep 2008 13:53:29 -0400 From: "Robert Richmond" <[EMAIL PROTECTED]> Subject: [Histonet] Re: Humidity levels in the lab To: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=ISO-8859-1 >>In the summertime [the humidity in our laboratory] is at around >>60-70%, which seems to work well. In the winter time it is less than 0 >>and I run a humidifier full out to try to achieve 20%.<< If your relative humidity is less than zero, you're probably putting too much thiotimoline in your water bath! (If you're too young for this literary allusion, see http://en.wikipedia.org/wiki/Thiotimoline ) and make the acquaintance of the late great Isaac Asimov! Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Mon, 22 Sep 2008 14:04:56 -0400 From: "Jerry Helisek" <[EMAIL PROTECTED]> Subject: [Histonet] RE: Oversized microscope supply source (IN USA) To: <histonet@lists.utsouthwestern.edu> Cc: [EMAIL PROTECTED] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" We also supply many larger sizes, see www.ralamb.com Thanks, ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America [EMAIL PROTECTED] 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Monday, September 22, 2008 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [EMAIL PROTECTED] You can reach the person managing the list at [EMAIL PROTECTED] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Oversized microscope slide source ([EMAIL PROTECTED]) 2. Microtome micron calibration and validation ([EMAIL PROTECTED]) 3. Beta-Gal antibody (Dustin Hambright) 4. CE opportunity (Jennifer MacDonald) 5. RE: Humidity levels in the lab (Burton, Lynn) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 14:06:57 -0400 From: [EMAIL PROTECTED] Subject: [Histonet] Oversized microscope slide source To: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED] ov> Content-Type: text/plain; charset=US-ASCII Here's a website for a company based in California, US. http://www.tedpella.com/histo_html/slides-large.htm The UK distributor is: Agar Scientific Ltd 66a Cambridge Road Stansted, Essex CM24 8DA United Kingdom Tel.: 44-1279-813519 [EMAIL PROTECTED] http://www.agarscientific.com Dodi Borsay Horowitz, Biologist US Environmental Protection Agency Atlantic Ecology Division 27 Tarzwell Drive Narragansett, Rhode Island 02882 401/782-3042 [EMAIL PROTECTED] ------------------------------ Message: 2 Date: Fri, 19 Sep 2008 11:19:08 +0530 From: [EMAIL PROTECTED] Subject: [Histonet] Microtome micron calibration and validation To: Histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 3 Date: Mon, 22 Sep 2008 11:44:22 -0400 From: Dustin Hambright <[EMAIL PROTECTED]> Subject: [Histonet] Beta-Gal antibody To: <histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="US-ASCII" Hello, Does anyone know a reliable source for a Beta-Gal (lac-z) antibody for mouse frozen immunohistochemistry? Do you know the company and dilution? Thank you in advance, Dustin ------------------------------ Message: 4 Date: Mon, 22 Sep 2008 09:18:43 -0700 From: Jennifer MacDonald <[EMAIL PROTECTED]> Subject: [Histonet] CE opportunity To: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="US-ASCII" Continuing Education Opportunity (3 CE units) Diagnostic BioSystems is sponsoring a workshop in cooperation with the California Society for Histotechnology. The workshop will be held at Mt. San Antonio College. Date: Saturday, October 18 Time: 9:30 AM to 1:00 PM Title: IHC: Basics and Beyond Maximum attendance: 60 For more information or to RSVP, please contact Jennifer MacDonald at [EMAIL PROTECTED] ------------------------------ Message: 5 Date: Mon, 22 Sep 2008 11:38:32 -0500 From: "Burton, Lynn" <[EMAIL PROTECTED]> Subject: RE: [Histonet] Humidity levels in the lab To: "Thomas Jasper" <[EMAIL PROTECTED]>, "Randolph-Habecker, Julie" <[EMAIL PROTECTED]> Cc: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="iso-8859-1" In the summertime ours is at around 60-70%, which seems to work well. In the winter time it is less than 0 and I run a humidifier full out to try to achieve 20%. It is definitely easier in the summer. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: [EMAIL PROTECTED] on behalf of Thomas Jasper Sent: Fri 9/19/2008 5:01 PM To: Randolph-Habecker, Julie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Humidity levels in the lab Julie, I can't offer you any references, but here's something to consider. A cool mist humidifier in your lab. We are in Bend, OR., on the dry side of the Cascades. Running the humidifier reduces static and makes sectioning a bit easier. No hard science with this, just a little more atmospheric moisture. By the way, the humidifier I bought is a big blue and white penguin. The mist streams out the beak. Just looking at it makes you smile. I bought it at Target for ~ $40.00 - it works for us. Have a good weekend. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 [EMAIL PROTECTED] -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Randolph-Habecker, Julie Sent: Thursday, September 18, 2008 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels in the lab Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 [EMAIL PROTECTED] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 26 **************************************** ------------------------------ Message: 4 Date: Mon, 22 Sep 2008 14:54:20 -0500 From: Jackie M O'Connor <Jackie.O'[EMAIL PROTECTED]> Subject: [Histonet] Histology schools/training in San Antonio, TX To: "histonet" <histonet@lists.utsouthwestern.edu>, [EMAIL PROTECTED] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="US-ASCII" Can someone advise me of the schools available in the San Antonio area, and what the prerequisites are? Thanks, Jackie ------------------------------ Message: 5 Date: Mon, 22 Sep 2008 16:28:07 -0400 From: [EMAIL PROTECTED] Subject: [Histonet] Microtome To: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Hello, We will be purchasing another microtome and I wanted to get feedback from the list.? If you wouldn't mind giving me your thoughts on the microtome you like the best.? We are not getting an automated one, not in our budget, but a simple, ever-lasting one.? We have the Leica 2135 and 2125 and sometimes the blade holder gives us problems, but I'm sure they all do.? Actually, we do have a Microm 330 and that one hasn't given us any problems at all. I just would like to know the one that gets a lot of positive feedback. Thanks so much in advance, Stacey, HT Biomed?Group ------------------------------ Message: 6 Date: Mon, 22 Sep 2008 16:30:48 -0400 From: "Owen, Michael P" <[EMAIL PROTECTED]> Subject: [Histonet] Seattle-Tacoma craigslist: Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" seattle-tacoma craigslist / jobs / biotech/sci http://seattle.craigslist.org http://seattle.craigslist.org/see/sci/850615554.html Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) ------------------------------------------------------------------------ -------- Reply to: see below Date: 2008-09-22, 9:23AM PDT Would you like to work for a premier provider of innovative solutions in drug discovery and development? MDS Pharma Services has an exciting opportunity as a Manager/Senior Manager, Histology and Laboratory Resources at our Bothell, WA location. In this full-time position, you will be responsible for the management of our histology and bioassay laboratory and staff in support of preclinical efficacy pharmacology studies. The job is expected to be split approximately 70% managerial functions and 30% histology or bioassay laboratory bench work. Technical experience in the preparation of paraffin, plastic and/or frozen tissue specimens is required. As the laboratory manager, you will develop and administer budgets, performance requirements, and schedules. You will also monitor and control labor and capital expenditures and will interpret results of laboratory activities. You will provide operational oversight of the laboratory and technical guidance with regard to feasibility and timing to project teams. Other staff functions include interviewing, hiring, and training employees as well as addressing staff complaints and oversight of the development of new methods or assays. A BS/BA degree in a scientific field and 8+ years of experience is desired along with 2+ years of supervisory/management experience. Equivalent education and experience is also acceptable. Experience in histology and /or, histomorphometry is required. Experience with ELISA, RIA or clinical pathology laboratory procedures are highly desirable. Experience in working skeletal or CNS diseases is a plus. Familiarity with GLP and CLIA requirements are desirable as well. AA/EEO Please apply on-line at www.mdsps.com. Location: Bothell, WA Compensation: Great pay and benefits! Principals only. Recruiters, please don't contact this job poster. Please, no phone calls about this job! Please do not contact job poster about other services, products or commercial interests. PostingID: 850615554 Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: [EMAIL PROTECTED] ------------------------------ Message: 7 Date: Mon, 22 Sep 2008 17:00:37 -0700 (PDT) From: Joseph Saby <[EMAIL PROTECTED]> Subject: Re: [Histonet] Cassette Holders To: [EMAIL PROTECTED], Histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=iso-8859-1 Jennifer- Be of good cheer! I saw them at the NSH convention. Now if I could only remember where... Joe ----- Original Message ---- From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]> To: Histonet@lists.utsouthwestern.edu Sent: Thursday, September 18, 2008 3:47:34 PM Subject: [Histonet] Cassette Holders Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 22 Sep 2008 17:11:33 -0700 (PDT) From: Jon Google <[EMAIL PROTECTED]> Subject: [Histonet] BioCare To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=iso-8859-1 Did anyone see the new biocare IHC stainer at NSH? could you give your impressions? We are looking at the Bond, Leica Bond, but we have just heard about the biocare system. Thanks in advance, ------------------------------ Message: 9 Date: Mon, 22 Sep 2008 20:33:18 -0400 From: Roxanne Soto <[EMAIL PROTECTED]> Subject: Re: [Histonet] BioCare To: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes This happens to be my favorite of all the newer instruments. If it does everything proposed-wow, what a time saver it would be. To be able to load it without the other program being finished. DAKO tried an instrument like this a couple of years ago but it bombed. I would set yourself up for a demo. Once they are released they are going to go quick- we are getting 4 of them throughout our system. Sent from my iPhone On Sep 22, 2008, at 8:11 PM, Jon Google <[EMAIL PROTECTED]> wrote: > Did anyone see the new biocare IHC stainer at NSH? could you give > your impressions? We are looking at the Bond, Leica Bond, but we > have just heard about the biocare system. > > Thanks in advance, > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 23 Sep 2008 14:02:15 +1000 From: "Orlowski Christian" <[EMAIL PROTECTED]> Subject: [Histonet] Cryosectioning dorsal mouse skin To: <histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Greetings all, I'm wondering whether there are any recommendations for how to effectively cryosection dorsal mouse skin for immunohistochemistry. I'm having difficulty with much of the mouse skin slipping off the slide during the fixation and washing steps, which seems to result in loss of the epidermis in particular, that part of the tissue that I happen to be interested in. I apply the dorsal mouse skin cryosections onto SuperFrost Plus (Menzel-Glaser) slides and air dry them for 2 hours. I use acetone for fixation, 10 minutes, and use PBS for washing. Any suggestions? This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. ------------------------------ Message: 11 Date: Tue, 23 Sep 2008 03:37:21 -0700 (PDT) From: Sohail Ejaz <[EMAIL PROTECTED]> Subject: [Histonet] detection of brain infarcts To: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=iso-8859-1 HI Everybody I am trying to find some stain that can detect brain infarct. Plreviously i was using TTC, but now we have ban on that. So, looking forward for a best alternative. Thanks Sohail ------------------------------ Message: 12 Date: Tue, 23 Sep 2008 12:07:08 GMT From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]> Subject: [Histonet] Re: Blind Review Policy To: [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=windows-1252 Does anyone have a Blind Case review Policy they could share with me? This is where when there is only one Pathologist and he does not have another pathologist to do peer review,In a one Pathologist lab. Is anyone else doing this? Thank you. Marsha Price ____________________________________________________________ Click to consolidate your debt in minutes, stop late or over-limit fees, pay less. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3m2bkZgujH4vUMxAF4GxmsePRHuCIW6Y6ywMqxb9PWv5ADBn/ ------------------------------ Message: 13 Date: Tue, 23 Sep 2008 08:37:15 -0500 From: "Ford Royer" <[EMAIL PROTECTED]> Subject: RE: [Histonet] Cassette Holders To: <Histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="iso-8859-1" I am not sure if this is what you are looking for but it is the one that I am familiar with that is still being manufactured. http://www.innovativelabacrylics.com/products/pages/cassette.html Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Joseph Saby Sent: Monday, September 22, 2008 7:01 PM To: [EMAIL PROTECTED]; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette Holders Jennifer- Be of good cheer! I saw them at the NSH convention. Now if I could only remember where... Joe ----- Original Message ---- From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]> To: Histonet@lists.utsouthwestern.edu Sent: Thursday, September 18, 2008 3:47:34 PM Subject: [Histonet] Cassette Holders Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 23 Sep 2008 06:58:05 -0700 From: "Trajkovic, Dusko" <[EMAIL PROTECTED]> Subject: [Histonet] Effect of 5%Aeti acid on Nuclei To: <Histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Good day to Everyone and for those that attended the NSH, WELCOME BACK. I would hope that the parties were good and I am truly sorry that I had to miss all of them. I have a friend who asked me to post this for him. Any answer or suggestion would be appreciated. Thank you Dusko Trajkovic I'm asking because in a paper I'm reviewing a paper where someone is using a concentration of 45-60% Acetic acid to fix isolated nuclei, then fixing again with Carnoy's, and then doing FISH (fluorescence in situ hyb) on them. I'm trying to figure out the effects on chromatin...does it cause the nuclei and/or chromatin to swell? I'd appreciate if you could ask around. Thanks, Yun ------------------------------ Message: 15 Date: Tue, 23 Sep 2008 07:11:22 -0700 (PDT) From: Rene J Buesa <[EMAIL PROTECTED]> Subject: Re: [Histonet] Effect of 5%Aeti acid on Nuclei To: Histonet@lists.utsouthwestern.edu, "Trajkovic, Dusko" <[EMAIL PROTECTED]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=iso-8859-1 Yes, the immediate effect of acetic is nuclear swelling. René J. --- On Tue, 9/23/08, Trajkovic, Dusko <[EMAIL PROTECTED]> wrote: From: Trajkovic, Dusko <[EMAIL PROTECTED]> Subject: [Histonet] Effect of 5%Aeti acid on Nuclei To: Histonet@lists.utsouthwestern.edu Date: Tuesday, September 23, 2008, 9:58 AM Good day to Everyone and for those that attended the NSH, WELCOME BACK. I would hope that the parties were good and I am truly sorry that I had to miss all of them. I have a friend who asked me to post this for him. Any answer or suggestion would be appreciated. Thank you Dusko Trajkovic I'm asking because in a paper I'm reviewing a paper where someone is using a concentration of 45-60% Acetic acid to fix isolated nuclei, then fixing again with Carnoy's, and then doing FISH (fluorescence in situ hyb) on them. I'm trying to figure out the effects on chromatin...does it cause the nuclei and/or chromatin to swell? I'd appreciate if you could ask around. Thanks, Yun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 23 Sep 2008 14:47:51 GMT From: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]> Subject: Re: [Histonet] Re: Blind Review Policy To: [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=windows-1252 Does anyone have a policy for a Blind Case Review for a one pathologist private lab? Thank you. Marsha Price ____________________________________________________________ Internet Security Software - Click here. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3mEWrnsLu04AAPUy8TIFwZG9ifrjPeX3laAPLUa845TgE3Sp/ ------------------------------ Message: 17 Date: Tue, 23 Sep 2008 11:53:17 EDT From: [EMAIL PROTECTED] Subject: Re: [Histonet] Cassette Holders To: [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="US-ASCII" Hello Jennifer; In response to your search for cassette holders, please check out this link: _http://www.hackerinstruments.com/labstorage.htm_ (http://www.hackerinstruments.com/labstorage.htm) They were on display at the NSH meeting in Pittsburgh. Best regards, Elfi Hacker In a message dated 9/18/2008 3:48:03 P.M. Eastern Daylight Time, [EMAIL PROTECTED] writes: Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001) ------------------------------ Message: 18 Date: Tue, 23 Sep 2008 12:48:14 -0400 From: "Joyce Cline" <[EMAIL PROTECTED]> Subject: RE: [Histonet] Ventana Retic Stain To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" We have to let our retic slides dry for 1 hour at 72C. We use superfrost plus slides otherwise we have variable staining or no staining. -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Laurie Colbert Sent: Thursday, September 18, 2008 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Retic Stain Is anyone out there having problems with their Retic stain on the Ventana Nexus Special Stainer? Please contact me offline. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 27 **************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
