[Histonet] RE: Bubble problems

2014-08-08 Thread Wolfe, Christina
Hi Melissa, We have intermittently had problems with our Leica Glass coverslipper over the years. During troubleshooting with their tech support, we found our issue was most likely with the coverslips. We use the VWR micro glass 24x50 mm cat# 48404-452 coverslips and the Tissue-Tek Glas mounti

[Histonet] RE: Bubble problems!

2014-08-07 Thread Melissa Ng
ern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, August 07, 2014 8:12 AM To: Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bubble problems! Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium

[Histonet] RE: Bubble problems!

2014-08-07 Thread Horn, Hazel V
AM To: Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bubble problems! Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any ai

[Histonet] RE: Bubble problems!

2014-08-07 Thread Barbara Tibbs
Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Dia