Jeanne Clark, Histology/IHC, Stanford Hospital and Clinics asks:
In looking through old procedures, I have found several different 'solutions'
that have been used for fixation and decalcification of bone (particularly
bone marrow cores). I would very much appreciate hearing what people are
Ms. Clark
We are currently use 10% formic Acid in a microwave oven to decal ours for
about 2 and 1/2 hour after they were fix in 10% neutral buffer Formalin. We
were told that they are Ok for immuno-stains.
Thomas Huynh, HT( ASCP)
Clinical Histology Technician
Pathology/ Histology/ Bone Lab
Hi Becky:
How long is the specimen in formalin before you put it in Cal Rite decal?
Thanks.
Richard
Richard W. Cartun, Ph.D.
Director, Histology Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
