Andrea,Go for: Vector Blue (Vector Labs) or Permanent Blue (Diagnostic Biosystems) for AP activityVector Red or Permanent Red, also for AP activity. These two AP immunostaining methods can be combined in a sequential double staining method with a HIER step (10 min, 98C) in between for removing the immunoreagents. Both red or blue reaction products survive the HIER step without damage (see JOH 31:119-127). Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 26 Aug 2009 14:05:41 -0400 From: Andrea Hooper <anh2...@med.cornell.edu> Subject: [Histonet] Double HRP immunostains To: Histonet <histonet@lists.utsouthwestern.edu>
I do double HRP immunostains. Works well using DAB/AEC and True Blue ... but what are your favorite chromagens to use? Would love some feedback for inspiration. Andrea -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
