[Histonet] RE: Eliminating the edge effect in IHC/IF

2010-02-16 Thread Karen Cai
Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai k...@prosci-inc.com Usually that is the result of incomplete fixation. Check your fixation protocol. Ren� J.. --- On Mon, 1/11/10, Karen Cai k...@prosci-inc.com wrote: From: Karen Cai k

[Histonet] RE: Eliminating the edge effect in IHC/IF

2010-01-12 Thread C.M. van der Loos
: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai k...@prosci-inc.com Usually that is the result of incomplete fixation. Check your fixation protocol. Ren� J. --- On Mon, 1/11/10, Karen Cai k...@prosci-inc.com wrote: From: Karen Cai k...@prosci

RE: [Histonet] RE: Eliminating the edge effect in IHC/IF

2010-01-12 Thread Sherwood, Margaret
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of C.M. van der Loos Sent: Tuesday, January 12, 2010 2:55 PM To: histonet@lists.utsouthwestern.edu Cc: k...@prosci-inc.com Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have

RE: [Histonet] RE: Eliminating the edge effect in IHC/IF

2010-01-12 Thread Rene J Buesa
Again, provided that you are doing IHC manually. René J. --- On Tue, 1/12/10, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF To: C.M. van der Loos c.m.vanderl...@amc.uva.nl

[Histonet] RE: Eliminating the edge effect in IHC/IF

2010-01-12 Thread Rene J Buesa
Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone:  +31 20 5665631   From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF To: histonet@lists.utsouthwestern.edu, Karen Cai k...@prosci-inc.com Usually that is the result of incomplete fixation. Check

RE: [Histonet] RE: Eliminating the edge effect in IHC/IF

2010-01-12 Thread Della Speranza, Vinnie
Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF Hi all,We also have observed this phenomenon many times. But sorry Colleen and Rene, I don't believe that an fixation issue is the explanation why the edges are sometimes stronger than the rest. To my opinion this is a bit too easy

RE: [Histonet] RE: Eliminating the edge effect in IHC/IF

2010-01-12 Thread Hermina Borgerink
-inc.com Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF Again, provided that you are doing IHC manually. René J. --- On Tue, 1/12/10, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: RE: [Histonet] RE: Eliminating the edge