Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu, Karen Cai k...@prosci-inc.com
Usually that is the result of incomplete fixation. Check your fixation protocol.
Ren� J..
--- On Mon, 1/11/10, Karen Cai k...@prosci-inc.com wrote:
From: Karen Cai k
: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu, Karen Cai k...@prosci-inc.com
Usually that is the result of incomplete fixation. Check your fixation protocol.
Ren� J.
--- On Mon, 1/11/10, Karen Cai k...@prosci-inc.com wrote:
From: Karen Cai k...@prosci
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of C.M. van der
Loos
Sent: Tuesday, January 12, 2010 2:55 PM
To: histonet@lists.utsouthwestern.edu
Cc: k...@prosci-inc.com
Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF
Hi all,We also have
Again, provided that you are doing IHC manually.
René J.
--- On Tue, 1/12/10, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF
To: C.M. van der Loos c.m.vanderl...@amc.uva.nl
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
phone: +31 20 5665631
From: Rene J Buesa rjbu...@yahoo.com
Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu, Karen Cai k...@prosci-inc.com
Usually that is the result of incomplete fixation. Check
Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF
Hi all,We also have observed this phenomenon many times. But sorry Colleen and
Rene, I don't believe that an fixation issue is the explanation why the edges
are sometimes stronger than the rest. To my opinion this is a bit too easy
-inc.com
Subject: RE: [Histonet] RE: Eliminating the edge effect in IHC/IF
Again, provided that you are doing IHC manually.
René J.
--- On Tue, 1/12/10, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: RE: [Histonet] RE: Eliminating the edge