Mike,
For EM work we fix, scrape the plate into a microtube, centrifuge at 1800 rpm
for 10 min and then embed in Histogel or agar. Processing after that is as
usual. They look fine by EM. I'm sure they would look fine by paraffin
processing as well.
Tim Morken
Supervisor, Electron Microscopy
We do this all the time. Here is our protocol:
Agarose Pre-embedding Cell Cultures Pellets Collected in 15 ml. Tubes
Material:
• 10% Neutral Buffer Formalin
• 1X Dulbeccos PBS
• NuSieve GTG Low Melting Point Agarose: Lonza, Cat# 50080
• Corning 15 ml conical Centrifuge
