I concur on this.
If my boss will let me, I can send you our elaborate protocol for H&E
staining, It involves many dishes and many washes. But your H&E will be
perfect.
I don't have the protocol with me right now,as I'm at home but tomorrow I
will ask about sending you the protocol. I don't see
Our protocol is to:
Use a positively charged slide
Fix in 10% formalin for 1 minute
Rinse in tap (a few dips)
Gill 3 for 1 minute
Rinse in tap (a few dips)
3-5 dips in bluing
Rinse in tap (a few dips)
Eosin 2-3 dips
100% ETOH (a few dips)
100% ETOH (a few dips)
Xylene (dip until clear)
Mount with
