Our software (NiceLabel / NiceWatch) has the ability to take in data from any system. Data can be text, XML, HL7, JOB files, open formatted files, etc. We can connect via TCP/IP, Web Services, COM Port or a file drop. The data is them parsed out and place onto a label template formatted in NiceLabel Pro. A GUI design tool. We support all barcode symbologies (1D and 2D). We can then output to any printer that has a Windows print driver. Thermal, Laser, Line Matrix, Ink Jet or devices like slide and cassette printers. Please contact me if you would like additional information, a demo or trial of our software.
Thank you, Maggie Allen Healthcare Business Development Manager Niceware International, LLC Tel (810) 629-3930 Cell (215) 200-0268 Email: maggie.al...@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 Corporate Numbers : General: (262) 784-2456 Toll Free: (888) 894-NICE (6423) Fax: (262) 784-2495 Technical Support: (262) 784-2466 Email: maggie.al...@nicewareintl.com www.nicewareintl.com http://healthcare.nicewareintl.com FREE Webinars : September 17, 2010 - 10:30AM CST - 11:30AM CST Learn How to Recognize NiceForm Application Opportunities Register Today! September 24, 2010 - 10:30AM CST - 11:30AM CST High Quality Color Labeling with NiceLabel Register Today! "The information in this e-mail and any attachments is confidential and may be subject to legal professional privilege. It is intended solely for the attention and use of the named addressee(s). If you are not the intended recipient or person responsible for delivering this information to the intended recipient, please notify the sender immediately. Unless you are the intended recipient or his/her representative you are not authorized to, and must not, read, copy, distribute, use or retain this message or any part of it" -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, September 09, 2010 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 82, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. H&E+ Alcian Blue (Deborah Faichney) 2. RE: bar coding specimens, slides, blocks (Tom McNemar) 3. FW: [Histonet] Odd question (Morken, Tim) 4. RE: bar coding specimens, slides, blocks (Feher, Stephen) 5. RE: Pathology billing for consultation (Feher, Stephen) 6. Re: FW: [Histonet] Odd question (kim.dona...@bhcpns.org) 7. giving back tissue (Tench, Bill) 8. Re: bar coding specimens, slides, blocks (Langenberg, Stacey) 9. HT position in Colorado (Hale, Meredith) 10. tissue refixation ? (Benoit Loup) 11. Re: H&E+ Alcian Blue (Bryan Llewellyn) ---------------------------------------------------------------------- Message: 1 Date: Thu, 9 Sep 2010 09:44:39 +0100 From: Deborah Faichney <d.a.faich...@stir.ac.uk> Subject: [Histonet] H&E+ Alcian Blue To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8ed3f2ca5b78e142b8193376c57330f8eae9644...@exch2007.ad.stir.ac.uk> Content-Type: text/plain; charset="us-ascii" Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. ------------------------------ Message: 2 Date: Thu, 9 Sep 2010 06:04:06 -0400 From: Tom McNemar <tmcne...@lmhealth.org> Subject: [Histonet] RE: bar coding specimens, slides, blocks To: "Horn, Hazel V" <hor...@archildrens.org>, "histo...@pathology.swmed.edu" <histo...@pathology.swmed.edu> Message-ID: <e9a90e28259d2f4e84308c5e8ea8f7b4a51d7...@lmhs-exchange.lmhealth.org> Content-Type: text/plain; charset="us-ascii" Also interested in this... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: histo...@pathology.swmed.edu Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. 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Thank you. ------------------------------ Message: 3 Date: Thu, 9 Sep 2010 08:48:05 -0700 From: "Morken, Tim" <timothy.mor...@ucsfmedctr.org> Subject: FW: [Histonet] Odd question To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1aaf670737f193429070841c6b2add4c0255010...@exmbmcb15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" <timothy.mor...@ucsfmedctr.org> 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'kim.dona...@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kim.dona...@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 9 Sep 2010 12:03:55 -0400 From: "Feher, Stephen" <sfe...@cmc-nh.org> Subject: RE: [Histonet] bar coding specimens, slides, blocks To: "Horn, Hazel V" <hor...@archildrens.org>, <histo...@pathology.swmed.edu> Message-ID: <73a7ed895ee0c24d9267ed814911df191773f...@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: histo...@pathology.swmed.edu Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 9 Sep 2010 12:06:21 -0400 From: "Feher, Stephen" <sfe...@cmc-nh.org> Subject: RE: [Histonet] Pathology billing for consultation To: "Demarinis, Carolyn" <cdemari...@saratogacare.org>, <histo...@pathology.swmed.edu> Message-ID: <73a7ed895ee0c24d9267ed814911df191773f...@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" There are a couple of ways to handle this and most of them have already been mentioned. We have a contract Pathology group in our hospital so if the patient insurance does not pay, the consultant bills the Pathology Group. Unless your pathologists are completely sold on their expert consultants, outside labs such as Genzyme will do the third party billing if you use their consultants. I know that they accept insurance assignment and have relationships with most large insurers so the patient rarely gets stuck with a bill. Steve -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, September 08, 2010 9:22 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Pathology billing for consultation I would like to know how other pathology labs are billing for consultations that are sent out by pathologist for second opinion. Our process is to notify the physician's office that a case is being sent to an expert and, if required, the physician's office is responsible for obtaining precertification if the patient's insurance require it. Unfortunately, this has caused us a number of problems. If the consultant is not "in-network", the insurance does not cover this expense, and the patient is responsible for the bill. Is it a better option for the hospital to receive all bills from consultants, and in turn, the hospital will bill the patient? If so, are there problems associated with this? Or are other laboratories having the consultants bill the patient's insurance directly, and if so, are they experiencing similar problems? Thanks. Carolyn DeMarinis, Pathology Supervisor Saratoga Hospital Laboratory This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at priv...@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 9 Sep 2010 11:12:55 -0500 From: kim.dona...@bhcpns.org Subject: Re: FW: [Histonet] Odd question To: "Morken, Tim" <timothy.mor...@ucsfmedctr.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of9458e274.25ca7447-on86257799.0059060a-86257799.00591...@bhcpns.org> Content-Type: text/plain; charset="US-ASCII" Thank you all for responding. All of you were very helpful. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Morken, Tim" <timothy.mor...@ucsfmedctr.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 09/09/2010 10:48 AM To "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> cc Subject FW: [Histonet] Odd question This link will take you to the article about legalisms of tissue and blocks: http://labmed.ascpjournals.org/search?fulltext=tissue+blocks&submit=yes&x=18&y=12 Tim >>> "Morken, Tim" <timothy.mor...@ucsfmedctr.org> 9/8/2010 6:49 PM >>> There was a good article about this topic in Lab Medicine (ASCP) "Who Owns Diagnostic Tissue Blocks?" February 2009, Vol 40, No 2, pf 69-73 (available in pdf format on line at www.ascp.org). It also addresses wet tissue, other things taken out of people. The key sentence in that article is: "In practice, there are no specific laws, case law, or prior legal rulings that explicitly address ownership of diagnostic materials." So, it is left up to individual institutions to develop their own guidelines. Obviously, then, there will be many variations of practice. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 08, 2010 3:18 PM To: 'kim.dona...@bhcpns.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Odd question Consult your medical legal department on this issue because this is not new situation. It is often times based on the person religion but the request should be made know at the time of surgery. So pathology can be made aware and avoid putting the specimen in formalin. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kim.dona...@bhcpns.org Sent: Wednesday, September 08, 2010 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Odd question Hi Histonetters, Can anyone give me any idea of any laws that guide giving a patient there organ after we have taken from it what we need to do the Histology? I know we have to keep it for a minimum of two weeks after sign out ( our policy is 6 weeks after sign out ). But then we dispose of it as medical waste. Are any of you aware of any guidelines on giving a patient there entire organ which would be submerged in formalin? Help and thanks in advance :-) Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 9 Sep 2010 09:14:12 -0700 From: "Tench, Bill" <bill.te...@pph.org> Subject: [Histonet] giving back tissue To: histonet@lists.utsouthwestern.edu Message-ID: <2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local> Content-Type: text/plain; charset=us-ascii In all of this discussion, it is important to understand that there are significant variations in state laws that relate to this issue, so if this problem arises, have your hospital/lab attorney check into state laws very carefully. In California, the laboratory may not "own" the tissue (again, depending on releases never read but signed by the patient on admission) but it clearly is the responsible "custodian." This applies to sending out slides and blocks for research and for clinical testing. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 8 Date: Thu, 9 Sep 2010 10:16:11 -0600 From: "Langenberg, Stacey" <stacey.langenb...@ucdenver.edu> Subject: Re: [Histonet] bar coding specimens, slides, blocks To: "Feher, Stephen" <sfe...@cmc-nh.org>, "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu>, "Horn, Hazel V" <hor...@archildrens.org>, "histo...@pathology.swmed.edu" <histo...@pathology.swmed.edu> Message-ID: <99316908.1375278.1284048976798.javamail....@bda2340.bisx.prod.on.blackberry> Content-Type: text/plain; charset="iso-8859-1" We too use both the IPS and IPC. These are on such an open system we created our own LIS and now have barcoding from grossing to slide signout. Stacey Sent via BlackBerry from T-Mobile -----Original Message----- From: "Feher, Stephen" <sfe...@cmc-nh.org> Sender: "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Date: Thu, 9 Sep 2010 10:03:55 To: Horn, Hazel V<hor...@archildrens.org>; histo...@pathology.swmed.edu<histo...@pathology.swmed.edu> Subject: RE: [Histonet] bar coding specimens, slides, blocks We have had a great deal of success using Leica's IPC and IPS cassette and slide labelers. We use Soft Path but I had heard that the Leica LIS experts have experience interfacing with a number of LIS systems. We set these up basically as just another printer that is interfaced with our LIS system. The software within the Leica labelers produces the cassettes and slides with any configuration of accession number and/or bar code that you would like. We chose to use 2d barcode to save space on the cassette and slide. We also print our ThinPrep slides this way. Leica and Hologic got together to make sure we were able to use the correct coding to use these slides on our ThinPrep Imager. Steve -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: histo...@pathology.swmed.edu Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 9 Sep 2010 11:21:24 -0500 From: "Hale, Meredith" <mh...@carisls.com> Subject: [Histonet] HT position in Colorado To: <histonet@lists.utsouthwestern.edu> Cc: "Roupp, Kevin" <kr...@carisls.com> Message-ID: <6f33d8418806044682a391273399860f051f3...@s-irv-ex301.pathologypartners.intranet> Content-Type: text/plain; charset="us-ascii" Colorado GI Pathology (CGIP) is seeking an experienced histotech to complement its existing staff. CGIP is a specialized lab located in Centennial, CO that provides services to the three largest gastroenterology practices in Denver. Dr. Russell Nash is the chief pathologist and medical director. Tracy Wrinkle is the Operations Manager. The position can be filled by an experienced person who can work from 32 to 40 hours per week. The schedule is basically days, although coverage for other times during vacations will be needed. The pay range starts at $26.00 per hour with benefits. This is a small, congenial work place with an emphasis on team work and cooperation. If you are interested in learning more about the opportunity, please contact Tracy Wrinkle at twrin...@cogipath.com or call her at (303) 770-4848 for a confidential interview and tour of the facility. Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mh...@carisls.com ------------------------------ Message: 10 Date: Thu, 09 Sep 2010 18:23:04 +0200 From: Benoit Loup <benoit.l...@jouy.inra.fr> Subject: [Histonet] tissue refixation ? To: histonet@lists.utsouthwestern.edu Message-ID: <4c8909e8.7040...@jouy.inra.fr> Content-Type: text/plain; charset=windows-1252; format=flowed Hi to all, I have some troubles with H/E staining of tissues fixed in Bouin's. In fact it worked very well during my first assays and now my tissue sections are torn and looks like bad. I think that the fixation time was not sufficient (4h for 3mmx2mmx2mm pieces). Thus is it possible to dewax, rehydrate and refix tissue in bouin's before sectioning ? I also have some pieces already sectioned and mounted on slide but not yet dewaxed and stained. Is it also possible to refix these sections or are they definitively lost ? Thanks to all for your comments and help. Benoit -- Benoit Loup, PhD UMR Biologie du D?veloppement et Reproduction Diff?renciation des Gonades et Perturbations INRA ? Domaine de Vilvert B?timent Jacques Poly 78350 Jouy en Josas France Tel: 33 1 34 65 25 38 Fax: 33 1 34 65 22 41 E-mail: benoit.l...@jouy.inra.fr ------------------------------ Message: 11 Date: Thu, 9 Sep 2010 09:43:03 -0700 From: "Bryan Llewellyn" <llewl...@shaw.ca> Subject: Re: [Histonet] H&E+ Alcian Blue To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <a9f1b4e2d2f9459ab21796c095110...@bryanpc> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original You are probably using a regressive hematoxylin. Most of those stain acid mucins purple-blue. To overcome it use a strictly progressive hematoxylin such as Mayer (hx 1 g., alum 50 g.) for 5 minutes. The order should be alcian blue, wash, H&E. Bryan Llewellyn ----- Original Message ----- From: "Deborah Faichney" <d.a.faich...@stir.ac.uk> To: <histonet@lists.utsouthwestern.edu> Sent: Thursday, September 09, 2010 1:44 AM Subject: [Histonet] H&E+ Alcian Blue Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it? For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 82, Issue 11 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
