Hi Kevin, I currently have the Leica IPS and IPC labeling system. There are some definitely pros/cons: Pros: Great for high volume Has convenient tray loading attachment (optional)
Cons: Takes up a lot of space Very particular for type of slides used (may be a issue for cost saving purposes) High costs for service plans, would have to compare prices to other models (pm's, maintenance etc) Cassettes sometimes create static through the printer and prevent from ejecting out resulting in jamming I have used this system in a low volume and high volume lab. They haven't given me too much trouble. The problems I have stated I have encountered within the 3 years I have been working with them. Let me know if you need more information! Alicia Lange Laboratory Supervisor University of Nevada, Reno Pathology Department arla...@medicine.nevada.edu ------------------------------ Message: 2 Date: Tue, 25 Oct 2011 13:52:28 -0400 From: "Kevin Reif" <kr...@stegh.on.ca> Subject: [Histonet] Hi Group: To: <histonet@lists.utsouthwestern.edu> Message-ID: <4ea6bf1d.2151.00b...@stegh.on.ca> Content-Type: text/plain; charset=US-ASCII Hi Group: Looking for opinions on cassette and slide labellers?? If you are using one please let me know the make, model and what you think of the machine, good or bad. Thanks in advance for all your input. Kevin Reif Senior Technologist - Pathology St. Thomas-Elgin General Hospital St. Thomas, On. Can. __________________________________________________________________________ St. Thomas Elgin General Hospital 189 Elm St. St. Thomas Ontario N5R 5C4 (519) 631-2020 http://www.stegh.on.ca Proud recipients of 2010 OHA Quality Healthcare Workplace Award - Platinum __________________________________________________________________________ Confidentiality/Privacy Notice: This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. For more information on our Privacy Policy, please see our website at http://www.stegh.on.ca ------------------------------ Message: 3 Date: Tue, 25 Oct 2011 10:58:04 -0700 (PDT) From: Steve Wong <swong...@yahoo.com> Subject: [Histonet] Please unsubscribe To: Histonet@lists.utsouthwestern.edu Message-ID: <1319565484.85941.yahoomailclas...@web65902.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello, Please unsubscribe me from this list. swong...@yahoo.com Thanks! ------------------------------ Message: 4 Date: Tue, 25 Oct 2011 12:59:41 -0500 From: "Harrison, Sandra C." <sandra.harris...@va.gov> Subject: [Histonet] saving slides between levels for IHC? To: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com>, "Horge, Pamela J" <phor...@fairview.org>, <rollmann.de...@mayo.edu> Cc: histonet@lists.utsouthwestern.edu Message-ID: <db425e28065da14faa75a282959620af05abd...@vhav23msga2.v23.med.va.gov> Content-Type: text/plain; charset="us-ascii" Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ------------------------------ Message: 5 Date: Tue, 25 Oct 2011 21:02:10 +0300 From: Badz Yahoo <badzros...@yahoo.com> Subject: [Histonet] EMS 9000 To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <80fd49ab-b170-4680-b36e-eeecd98f2...@yahoo.com> Content-Type: text/plain; charset=us-ascii Hi histonetts..Anyone using the microwave machine EMS 9000?I appreciate if someone can give me some feedback..tanx ------------------------------ Message: 6 Date: Tue, 25 Oct 2011 11:05:10 -0700 From: "Morken, Timothy" <timothy.mor...@ucsfmedctr.org> Subject: [Histonet] RE: saving slides between levels for IHC? To: "'Harrison, Sandra C.'" <sandra.harris...@va.gov>, "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com>, "Horge, Pamela J" <phor...@fairview.org>, "rollmann.de...@mayo.edu" <rollmann.de...@mayo.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8d7c2d242dbd45498006b21122072bf89448a...@mcinfrwem003.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Sandy, we have various protocols, some that have unstained between those to be stained. However, we have moved away from having "extras" cut "just in case" because we had thousands of unstained slides stored that were never used. After a few months they may be unreliable for staining. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 11:00 AM To: Webb, Dorothy L; Horge, Pamela J; rollmann.de...@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 25 Oct 2011 15:15:23 -0400 From: "Angela Bitting" <akbitt...@geisinger.edu> Subject: Re: [Histonet] saving slides between levels for IHC? To: "Pamela J Horge" <phor...@fairview.org>, "Dorothy L Webb" <dorothy.l.w...@healthpartners.com>, <rollmann.de...@mayo.edu>, "Sandra C. Harrison" <sandra.harris...@va.gov> Cc: histonet@lists.utsouthwestern.edu Message-ID: <4ea6d28b.2b7f.00c...@geisinger.edu> Content-Type: text/plain; charset=US-ASCII we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, liver core bxs, prostate needle core bxs. >>> "Harrison, Sandra C." <sandra.harris...@va.gov> 10/25/2011 1:59 PM >>> Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------ Message: 8 Date: Tue, 25 Oct 2011 21:44:11 +0100 (BST) From: Rhonda Henshall-Powell <rlhenshall_pow...@yahoo.co.uk> Subject: [Histonet] Seeking Field Application Specialists and Education Coordinator for CA-based Cancer Diagnostics Company To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <1319575451.47919.yahoomail...@web29501.mail.ird.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histonetters! Here I am again - An industry-leading IHC/ISH diagnostics company is seeking an in-house Education Coordinator and two Field Application Specialists (Midwest and Northwest) requiring significant travel: Field Applications Specialist Provide expert level technical support knowledge of Anatomic Pathology principles to troubleshoot and support instrumentation, software, and train lab personnel on use, optimisation and validation of IHC and ISH reagents. If interested or know of someone who may be interested, please reply directly to me (rlhenshall_pow...@yahoo.co.uk) for more information. Thank you Best Regards, Rhonda ------------------------------ Message: 9 Date: Tue, 25 Oct 2011 15:09:32 -0700 From: Patrick Laurie <foreig...@gmail.com> Subject: Re: [Histonet] saving slides between levels for IHC? To: Angela Bitting <akbitt...@geisinger.edu> Cc: Pamela J Horge <phor...@fairview.org>, rollmann.de...@mayo.edu, Dorothy L Webb <dorothy.l.w...@healthpartners.com>, histonet@lists.utsouthwestern.edu Message-ID: <cakeyg-2aevcrc9ijs+qshs1esmipvwwiuq8_gs0janbs2et...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We tend to take extras between levels especially on scant or needle biopsy tissue. We do waste several thousand slides per year, but by doing this it allows us to give the pathologist several representative sections and also save tissue for future IHC and special stains. On Tue, Oct 25, 2011 at 12:15 PM, Angela Bitting <akbitt...@geisinger.edu>wrote: > we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, > liver core bxs, prostate needle core bxs. > > >>> "Harrison, Sandra C." <sandra.harris...@va.gov> 10/25/2011 1:59 PM >>> > Does anyone know if it is standard to save sections from between the > levels for IP? Is it standard at your institution? > > > > > > Sandy C. Harrison, HTL (ASCP) > > Histology Supervisor > > Minneapolis VA > > 612-467-2449 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard > Protected Health Information and other confidential data contained in > external e-mail messages. If email is encrypted, the recipient will receive > an e-mail instructing them to sign on to the Geisinger Health System Secure > E-mail Message Center to retrieve the encrypted e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ------------------------------ Message: 10 Date: Wed, 26 Oct 2011 07:51:56 +0100 From: "Hobbs, Carl" <carl.ho...@kcl.ac.uk> Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <11d9615b89c10747b1c985966a63d7ca3826477...@kcl-mail04.kclad.ds.kcl.ac.uk> Content-Type: text/plain; charset="us-ascii" Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-)) Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity. In my experience. Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/ Whichever way you go....good luck. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 ------------------------------ Message: 11 Date: Wed, 26 Oct 2011 07:28:54 -0400 From: "Sheila Fonner" <sfon...@labpath.com> Subject: RE: [Histonet] saving slides between levels for IHC? To: "'Harrison, Sandra C.'" <sandra.harris...@va.gov>, <histonet@lists.utsouthwestern.edu> Message-ID: <000001cc93d2$71a7f670$54f7e350$@com> Content-Type: text/plain; charset="US-ASCII" Sandy, I don't think it is a "standard" procedure. However, our pathologist will frequently ask us to keep intervening levels for possible IHC on certain cases that are small or where the tumor is about to be exhausted. We are a dermatopathology lab. When it is requested, we cut the whole case on a clean waterbath, using distilled water and no additives, on plus slides. The routine H&E's are stained normally and the other slides are dried and held for possible immunos. Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 2:00 PM To: Webb, Dorothy L; Horge, Pamela J; rollmann.de...@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 26 Oct 2011 09:42:18 -0400 From: "Connolly, Brett M" <brett_conno...@merck.com> Subject: [Histonet] RE: Re: Seeking Beta-Amyloid not needing FA pretreatment To: "Hobbs, Carl" <carl.ho...@kcl.ac.uk>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c01c35b84dcdce49bc60867e87f1c8fe345dd4d...@usctmxp51015.merck.com> Content-Type: text/plain; charset="us-ascii" Interesting....we see APP on frozen sections with 6E10, but not in paraffin sections after citrate HIER. We use the monoclonal from Covance, 1:12000 overnight at 4C. Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Wednesday, October 26, 2011 2:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-)) Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity. In my experience. Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/ Whichever way you go....good luck. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 13 Date: Wed, 26 Oct 2011 08:49:26 -0500 From: Roger Brown <rbr...@mst.edu> Subject: [Histonet] Please remove my name To: <Histonet@lists.utsouthwestern.edu> Message-ID: <cacd7a16.92cd%rbr...@mst.edu> Content-Type: text/plain; charset="US-ASCII" Hello, Please remove my name from the Histonet mailing list. Thank you, Roger Brown (rbr...@mst.edu) ------------------------------ Message: 14 Date: Wed, 26 Oct 2011 09:56:15 -0400 From: "Vanessa J. Phelan" <vjp2...@columbia.edu> Subject: [Histonet] Please remove my name To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <cacd89bf.6cf5%phe...@icg.cpmc.columbia.edu> Content-Type: text/plain; charset="iso-8859-1" Hi, please remove my name from the mailing list. Thank you ------------------------------ Message: 15 Date: Wed, 26 Oct 2011 07:32:57 -0700 From: Bea DeBrosse-Serra <bdebrosse-se...@isisph.com> Subject: [Histonet] RE: Please remove my name To: "'Roger Brown'" <rbr...@mst.edu>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <493caa64f203e14e8823737b9ee0e25f091e912...@exchmb01.isis.local> Content-Type: text/plain; charset="us-ascii" If you want to be removed from the list, you have to do it yourself by going to the website below. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Brown Sent: Wednesday, October 26, 2011 6:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Please remove my name Hello, Please remove my name from the Histonet mailing list. Thank you, Roger Brown (rbr...@mst.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 26 Oct 2011 08:10:43 -0700 (PDT) From: Kim Donadio <one_angel_sec...@yahoo.com> Subject: Re: [Histonet] saving slides between levels for IHC? To: Sheila Fonner <sfon...@labpath.com>, "'Harrison, Sandra C.'" <sandra.harris...@va.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1319641843.94105.yahoomail...@web112302.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I think it depends on what the specimen is. All of the places Ive worked have always kept extras(levels) on sentinal nodes for keratins, also for prostate needle biopsies for p63/504s or other IHC's. Really doesnt it come down to what the Pathologist needs to make a diagnosis for the patient? I know it's a pain to cut so many levels and save them. Its also costly. But trust me, none of that is as bad as the sinking feeling you get when a biopsy has been exhausted and they need more stains :( Hope this helps. Just my 2 cents worth....... Kim Donadio ________________________________ From: Sheila Fonner <sfon...@labpath.com> To: "'Harrison, Sandra C.'" <sandra.harris...@va.gov>; histonet@lists.utsouthwestern.edu Sent: Wednesday, October 26, 2011 7:28 AM Subject: RE: [Histonet] saving slides between levels for IHC? Sandy, I don't think it is a "standard" procedure. However, our pathologist will frequently ask us to keep intervening levels for possible IHC on certain cases that are small or where the tumor is about to be exhausted. We are a dermatopathology lab. When it is requested, we cut the whole case on a clean waterbath, using distilled water and no additives, on plus slides. The routine H&E's are stained normally and the other slides are dried and held for possible immunos. Sheila KDL Pathology Knoxville, TN -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Tuesday, October 25, 2011 2:00 PM To: Webb, Dorothy L; Horge, Pamela J; rollmann.de...@mayo.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] saving slides between levels for IHC? Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 26 Oct 2011 12:00:59 -0400 From: "Sheila Fonner" <sfon...@labpath.com> Subject: [Histonet] EBER ISH To: <histonet@lists.utsouthwestern.edu>, "'Paul Googe'" <pgo...@labpath.com>, "'Susan Foreman'" <sfore...@labpath.com> Message-ID: <001901cc93f8$74289190$5c79b4b0$@com> Content-Type: text/plain; charset="us-ascii" Histonetters, Is anyone out there using the EBER ISH probes from Ventana on the Ultra Platform? I have a few questions. Did you need to get new software installed? Was there any training involved? And do you have successful protocols that you are willing to share? I have asked the questions before, but at the time there was nobody actually using it. I know that there were inquiries about the protocols because of the ASR status and the fact that Ventana cannot give you any advice. Any and all information would be greatly appreciated since we are wanting to start it up at our facility. Thanks, Sheila KDL Pathology Knoxville, TN ------------------------------ Message: 18 Date: Wed, 26 Oct 2011 12:04:28 -0400 From: "Sheila Fonner" <sfon...@labpath.com> Subject: [Histonet] Antibody questions To: <histonet@lists.utsouthwestern.edu> Message-ID: <001e01cc93f8$f085e120$d191a360$@com> Content-Type: text/plain; charset="us-ascii" Is there anyone who is doing myc, Bap1, Pten, or CD123 for dermpath specimens, and who is your vendor? Do you like the products, and what platform are you using? Thanks for any information you may have. These are new requests from our pathologist. Sheila ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 95, Issue 31 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet