Christina,
The following might be useful:
Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from
formalin fixed lung tissue that had previously been shown to be positive by
sputum culture. They were unable to culture TB from these tissues.
Gerston et al (HUM PATHOL
Hi,
Do anyone have references for how long should autopsy/necropsy tissue be in
fixation for a suspected TB case? Is 72-96 hours fixation sufficient or should
the time be extended to 5-7 days.
Thanks,
Christina Thurby
Bristol-Myers Squibb Company
Research Scientist I
812-307-2093 (tie 625)
As long as possible. I remember reading somewhere about TB in lung
tissue that was still viable after *years* of 10% NBF. Make sure the lungs
are thoroughly perfused, or they will still be unfixed in the middle. There
is a technique to inflate lungs at autopsy using a large syringe full of
5 days (96 h) is more than enough, but always handle the tissue and
blocks using safety precautions.
René J.
--- On Wed, 4/13/11, Thurby, Christina christina.thu...@bms.com wrote:
From: Thurby, Christina christina.thu...@bms.com
Subject: [Histonet] RE: How long should autopsy/necropsy tissue
Hi,
We fix 'Category-A' agent infected murine lungs in 10% buffered formalin for 7
days in ABSL-3. We also confirm tissue sterility by plating the tissue
homogenates on suitable media before taking the samples out of ABSL-3 facility
for sectioning. In your case 7 days should be enough, but it
