Michael: I use 1.5mm nichrome transfer loops for IHC staining of 50um
free floating sections and I move them into clean culture wells for each
solution. I purchase mine from Ted Pella. It does takes some practice
but the loops work quite well with minimal damage to the tissue.
Jean Mitchell,
: IHC free floating sections
Michael: I use 1.5mm nichrome transfer loops for IHC staining of 50um
free floating sections and I move them into clean culture wells for each
solution. I purchase mine from Ted Pella. It does takes some practice
but the loops work quite well with minimal
Apologies if I have not adhered to Histonet protocol: I have changed my Browser
from IE8 to latest Firefox and am not yet familiar!
I agree with Rene: stay with the same wells and give additional, longer washes.
Imho, even experienced users suffer section damage if they change wells.
