Hiro,
You are correct, alveolar macrophages do express less F4/80 than other tissue
macrophages. I refer you to, for example:
Zhang X, Goncalves R, Mosser DM. The isolation and characterization of murine
macrophages. Curr Protoc Immunol. 2008 Nov;Chapter 14:Unit 14.1. PubMed PMID:
19016445; PubMed
Hi. all
Let me add another question linked to marcophage staining.
So far we have done macrophage staining several times with F4/80, and
in most of the cases
IHC images were quite beautiful, for liver, spleen, adipose tissue and so on.
BUT, we have not succeeded in staining lung alveolar macrophage
Serotec has two antibodies that will work a F4/80 and CD68, both are rat
anti-mouse. We have used them in the same application that you will be using
(mouse aorta) with good success.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3
